Abcam, ab121244, Anti-RPL35 antibody

Size: 100µL
Rabbit Polyclonal RPL35 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human 60S ribosomal protein L35 aa 1 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human 60S ribosomal protein L35 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.P42766

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RPL35 also known as Ribosomal Protein L35 plays an essential role in ribosome function. It is a component of the large 60S ribosomal subunit and has a molecular mass of around 13.4 kDa. RPL35 exhibits widespread expression in human tissues suggesting it is necessary for general protein synthesis functions across different cell types.
Biological function summary
The function of RPL35 contributes to the formation and stability of the ribosome which is a complex structure composed of ribosomal RNA and ribosomal proteins. RPL35's integration into the ribosome assists in the assembly of new proteins by ensuring correct translation. This integration is necessary for maintaining the efficiency and accuracy of protein synthesis within cells playing an important role in cell growth and proliferation.
Pathways
The activity of RPL35 influences the mRNA translation pathway and connects closely to the protein biosynthesis process. Through its interaction with the ribosomal machinery it also relates to the mTOR signaling pathway which is significant for cell growth regulation. Proteins like RPLP0 and RPLP1 are associated with RPL35 within these pathways supporting a robust translation and protein synthesis network.
Mutations and dysregulation of RPL35 relate to disorders like Diamond-Blackfan anemia and some cancers. These conditions link to the disruption in ribosome biogenesis or function which leads to faulty protein synthesis. In Diamond-Blackfan anemia abnormal function of RPL35 along with other ribosomal proteins such as RPL5 disrupts normal erythropoiesis resulting in anemia.