Abcam, ab12157, Anti-PLK1 (phospho T210) antibody

Size: 100µg
Rabbit Polyclonal PLK1 phospho T210 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 5 publications.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Detects a band at 55kDa in mouse A20 cells that we believe corresponds to Plk1 (predicted molecular weight 68kDa). Also gives positive staining in immunohistochemistry in human colon carcinoma as expected. However, we have been unable to conclusively demonstrate the specificity of the antibody for phospho T210 Plk1.Anti-Polo-like Kinase pT210 Antibody is directed against human phosphorylated Plk1 protein. This antibody is specific for phosphorylated human Plk-1 protein at the pT210 residue. BLAST analysis indicates 100 % homology of the immunizing sequence with Plk-1 homologues from human, chimpanzee, pig, chicken, mouse, rat, Xenopus, dog, mosquito, zebra fish, starfish, sea urchin and fruit fly. Cross reactivity with Plk-1 protein homologues from C.elgans and honeybee may also occur as sequence homology varies by one amino acid residue in this sequence. Reactivity with Plk-1 protein from other sources is not known. Minimal reactivity is expected with the non-phosphorylated form of the protein.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-This product is directed against human phosphorylated Plk1 protein. This antibody was affinity purified., Storage buffer-pH: 7.2Preservative: 0.01% Sodium azideConstituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle