Abcam, ab121633, Anti-NSUN5 antibody

Size: 100µL
Rabbit Polyclonal NSUN5 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NSUN5 aa 100-250.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human NSUN5 aa 100-250. The exact immunogen used to generate this antibody is proprietary information.Q96P11

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NSUN5 also known as NOL1/NOP2/Sun domain family member 5 is a methyltransferase enzyme responsible for the methylation of cytosine residues in RNA. It has an estimated mass of approximately 50 kDa. Researchers have found NSUN5 expressed in various tissues with significant levels observed in the liver brain and reproductive tissues. Its primary mechanical function facilitates the post-transcriptional modification of RNA influencing RNA stability and translation efficiency.
Biological function summary
The methylation activity of NSUN5 contributes to cellular processes like RNA processing and gene expression regulation. NSUN5 forms part of the sub-complex associated with the large ribosomal subunit in eukaryotic cells. This connection suggests its role in ribosome biogenesis and protein synthesis. Studies also highlight NSUN5's involvement in safeguarding cell survival under stress by maintaining ribosomal RNA (rRNA) stability.
Pathways
NSUN5 operates as an integral component in the ribosome biogenesis pathway and the RNA modification pathway. It interacts with other proteins such as NSUN2 and NSUN6 which share similar RNA methylation functions. These interactions suggest a cooperative mechanism among NSUN family members in ensuring proper RNA and ribosome functionality important under both normal and stress conditions.
Researchers have linked NSUN5 to several health conditions including cancer and aging-related diseases. Elevated NSUN5 expression has been observed in various cancers proposing its role in tumorigenesis through dysregulation of cell growth control. Additionally its association with shortened human lifespan suggests an involvement in age-related changes through disrupted rRNA methylation. NSUN5's interaction with NSUN2 supports a complex network where alterations in RNA methylation contribute to disease development and progression.