Abcam, ab12172, Anti-HDAC4 antibody

Size: 50µL
Anti-HDAC4 antibody (ab12172) is a rabbit polyclonal antibody detecting HDAC4 in Western Blot, IP, ICC/IF . Suitable for Human, Mouse, Rat . - Over 30 publications - Trusted since 2004
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Rat, Mouse,
Applications:IP, WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human HDAC4 aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.P56524

Product details:
What is this antibody validated in?
Anti-HDAC4 antibody (ab12172) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.
What is the molecular weight of HDAC4?
Anti-HDAC4 (ab12172) specifically detects a band for HDAC4 (UniProt: P56524) at a molecular weight of 119kDa.
Trusted by the scientific community
Anti-HDAC4 (ab12172) was first used in a scientific publication in 2004 and has been cited over 30 times in peer-reviewed journals.
Reviewed by scientists
Anti-HDAC4 (ab12172) has over 5 independent reviews from customers.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-The antibody is affinity-purified using the immunizing peptide immobilized on agarose., Storage buffer-pH: 7.4Preservative: 0.097% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HDAC4 also known as Histone Deacetylase 4 functions in the regulation of gene expression through chromatin remodeling. This protein removes acetyl groups from histone proteins causing chromatin compaction and transcriptional repression. HDAC4 has a molecular weight of approximately 140 kDa. The protein is found in various tissues including brain heart and skeletal muscle and plays essential roles in various cellular processes. Alternate names for HDAC4 include KIAA0288 and HD4.
Biological function summary
The role of HDAC4 extends beyond chromatin remodeling as it serves as a critical regulator in several cellular functions. As part of a complex HDAC4 interacts with other proteins to control cell cycle differentiation and apoptosis. It cooperates with transcriptional repressors like MEF2 and RUNX2 influencing various signaling pathways. The protein's activity impacts neuronal survival muscle development and cardiac hypertrophy through its regulatory mechanisms.
Pathways
HDAC4 integrates into significant signaling networks notably the MAPK and calcium-calmodulin signaling pathways. Within the MAPK pathway HDAC4 associates with proteins like MEF2 impacting cellular growth and differentiation processes. In the calcium-calmodulin pathway HDAC4 affects gene transcription via its interaction with the phosphatase calcineurin linking intracellular calcium levels with transcriptional responses.
HDAC4 has associations with neurological disorders and cancer. Mutations and dysregulation in HDAC4 are linked to neuronal disorders such as Huntington's disease. In cancer HDAC4 interacts with other oncogenic proteins such as p53 influencing tumor progression and resistance to therapy. Consequently HDAC4 presents as a potential target for therapeutic intervention in these disease contexts.