Abcam, ab122241, Anti-ATP5I antibody

Size: 100µL
Rabbit Polyclonal ATP5I antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ATP5ME aa 1 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, ICC/IF, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human ATP5ME aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.P56385

Product details:
ab122241 is mono-specific.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ATP5I also known as ATP synthase subunit e is a part of the mitochondrial ATP synthase complex. Its molecular weight is approximately 11 kDa. ATP5I plays a role in the formation of the F1Fo ATP synthase which is present in the inner mitochondrial membrane. This complex is essential for the synthesis of ATP the energy currency of the cell. ATP5I is expressed in tissues with high energy demands such as heart and skeletal muscle where it contributes to energy production.
Biological function summary
ATP5I functions as part of the ATP synthase complex also known as Complex V within the mitochondria. This protein is involved in the final step of oxidative phosphorylation where it aids in the conversion of ADP to ATP using the proton gradient across the inner mitochondrial membrane. The presence of ATP5I ensures the proper assembly and function of the ATP synthase complex facilitating efficient production of ATP necessary for cellular activities.
Pathways
The involvement of ATP5I in oxidative phosphorylation emphasizes its key role in cellular metabolism. This process is integral to the electron transport chain a critical pathway for energy harvesting in cells. ATP5I works in concert with other subunits like ATP5F1 and ATP5H which together form the functional ATP synthase complex. The efficiency of this pathway directly affects energy production within cells linking ATP5I to metabolic regulation.
Aberrations in ATP5I function can contribute to mitochondrial diseases such as Leigh syndrome and mitochondrial encephalomyopathy. These disorders are characterized by defects in energy metabolism due to impaired ATP synthesis. Associations of ATP5I with other components of the ATP synthase complex like ATP5A1 suggest that disruptions in these relationships can lead to a cascade of dysfunctional metabolic activities highlighting its importance in maintaining cellular energy homeostasis.