Abcam, ab122427, Anti-NRAP antibody

Size: 100µL
Rabbit Polyclonal NRAP antibody. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NRAP.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human NRAP.Q86VF7

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The NRAP protein also known as Nebulin-Related Anchoring Protein serves as an essential component of muscle sarcomeres. It functions mechanically by anchoring nebulin filaments to the sarcomeric Z-lines contributing to the integrity of muscle fiber structure. The molecular weight of NRAP is approximately 197 kDa. This protein expresses highly in skeletal and cardiac muscles playing an important role in maintaining muscle architecture and function in these tissues.
Biological function summary
NRAP participates in muscle development and repair. It forms part of a protein complex that includes nebulin and actin filaments which collectively manage the assembly and stabilization of sarcomeres. Through its interactions with these filaments NRAP regulates the length of thin filaments during myofibrillogenesis. This protein facilitates effective muscle contraction and regeneration by maintaining the sarcomeric structure.
Pathways
NRAP integrates into significant muscle-related pathways such as the actin cytoskeleton signaling pathway and the sarcomere organization pathway. It interacts with proteins like talin and vinculin within these pathways which are fundamental for cytoskeletal stability and muscle cell adherence. These interactions help coordinate the structural assembly of sarcomeres ensuring proper muscle functionality and response to mechanical stress.
Alterations in NRAP expression or function have associations with certain muscle-related conditions including cardiomyopathy and muscular dystrophy. In the context of cardiomyopathy NRAP interacts with proteins like myopalladin a factor in cardiac myofibril integrity. In muscular dystrophy mutations affecting NRAP can lead to defects in muscle fiber formation and repair which further underline its connection to muscle disease pathophysiology.