Abcam, ab12251, Anti-Beta Lactamase antibody [8A5.A10]

Size: 500µg
Mouse Monoclonal Beta-lactamase antibody. Carrier free. Suitable for ELISA, WB and reacts with Escherichia coli samples. Cited in 33 publications.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:8A5.A10,
Isotype:IgG1,
Carrier free:Yes,
Reacts with:Escherichia coli,
Applications:WB, ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Specificity:This antibody specifically recognizes TEM type beta lactamases.

Product details:
Dilute in PBS or medium which is identical to that used in the assay system.
This product was changed from ascites to tissue culture supernatant on 19/12/2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions please do not hesitate to contact our scientific support team.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Purified from TCS, Storage buffer-pH: 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Beta-lactamase is an enzyme responsible for breaking down beta-lactam antibiotics such as penicillins and cephalosporins. This enzyme is also known by several names: beta lactamase beta-lactamase and b-lactamase. It has a molecular weight of approximately 29 kDa. Mainly beta-lactamase is expressed in various bacterial species including Escherichia coli and Staphylococcus aureus where it provides them with resistance against beta-lactam antibiotics. The presence of beta-lactamase significantly impacts the efficacy of these antibiotics in clinical settings.
Biological function summary
Beta-lactamase enzymes interact with beta-lactam antibiotics by hydrolyzing the beta-lactam ring within the antibiotic molecule. This action prohibits the antibiotics from binding to their bacterial targets rendering them ineffective. Beta-lactamase does not function alone; it can associate with other proteins in bacteria such as the outer membrane proteins which facilitate its secretion and activity. This enzyme is not part of a larger complex but works independently to confer antibiotic resistance to the host organism.
Pathways
Beta-lactamase plays a critical role in the antibiotic resistance pathway. This enzyme links to the cell wall biosynthesis pathway by inhibiting antibiotics that target bacterial cell wall synthesis. Because of this inhibition the production of cell wall components continues unhindered. Beta-lactamase activity is associated with penicillin-binding proteins (PBPs) which are the intended targets of beta-lactam antibiotics. By preventing the binding of antibiotics to these PBPs beta-lactamase ensures the survival of resistant bacterial strains.
Beta-lactamase contributes significantly to antibiotic resistance leading to infections that are difficult to treat such as methicillin-resistant Staphylococcus aureus (MRSA) and multidrug-resistant Escherichia coli infections. These bacteria produce beta-lactamase neutralizing the therapeutic effects of many commonly used antibiotics. The enzyme's action connects it to other proteins involved in resistance mechanisms like efflux pumps and proteases which also play parts in shielding bacteria from antibacterial agents. This correlation underlines the necessity for developing inhibitors that target beta-lactamase without hindering beneficial bacterial functions.