Abcam, ab123034, Anti-gamma Actin antibody [2A3]

Size: 100µL
Anti-gamma Actin antibody [2A3] (ab123034) is a mouse monoclonal antibody detecting gamma Actin in Western Blot, Flow Cytometry, IHC-P, IHC-Fr, ICC/IF, ELISA . Suitable for Chicken, Human, Mouse, Pig, Rabbit, Rat, Zebrafish . - Over 20 publications
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:2A3,
Isotype:IgG2b,
Carrier free:No,
Reacts with:Human, Pig, Chicken, Rat, Zebrafish, Mouse, Rabbit,
Applications:Flow Cyt, WB, ICC/IF, IHC-P, IHC-Fr, ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human ACTG1 aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.P63261,
Specificity:This antibody does not cross react with other actin isoforms. The epitope recognized by ab123034 is highly conserved and the antibody is therefore expected to react with many other species.This antibody is highly specific for gamma-cytoplasmic actin (γ-actin 1; encoded by ACTG1). It does not cross-react with the gamma-actin isoform expressed in the enteric smooth muscle tissues (γ-actin 2; encoded by ACTG2).

Product details:
What is this antibody validated in?
Anti-gamma Actin antibody [2A3] (ab123034) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA in Chicken, Human, Mouse, Pig, Rabbit, Rat, Zebrafish samples.
What is the molecular weight of gamma Actin?
Anti-gamma Actin [2A3] (ab123034) specifically detects a band for gamma Actin (UniProt: P63261) at a molecular weight of 42kDa.
Trusted by the scientific community
Anti-gamma Actin [2A3] (ab123034) was first used in a scientific publication in 2011 and has been cited over 20 times in peer-reviewed journals.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Storage buffer-Preservative: 0.09% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Gamma actin also known as actin gamma 1 and actin kDa is an essential actin protein found in the cytoskeleton. It is expressed widely in various tissues including muscle and non-muscle cells. This protein has a molecular mass of approximately 42 kDa. Gamma actin plays a mechanical role in maintaining cellular shape and facilitating cell motility by polymerizing into filamentous structures known as F-actin. It has alternate names such as 2a-3' and actin green in some contexts.
Biological function summary
Gamma actin contributes significantly to the structure of the cytoskeleton affecting cell shape and function. It forms part of actin filament complexes that provide tension and support to the cell. In both muscle and non-muscle cells gamma actin interacts with other actin isoforms to ensure filament stability and elasticity. These interactions are important for processes like cell division signal transduction and cellular transport.
Pathways
Gamma actin is involved in pathways related to cell motility and division. This protein is integral to the actin cytoskeleton pathway and has connections with proteins such as profilin and cofilin which regulate actin filament dynamics. Additionally it plays roles in signal transduction pathways that control cellular responses to external signals working alongside proteins like the Rho GTPases.
Gamma actin has been linked to conditions such as deafness and certain types of cancer. Mutations in the ACTG1 gene encoding gamma actin have been associated with hereditary hearing loss. In cancer changes in gamma actin expression can affect cell migration and invasion contributing to tumor progression. The protein's interaction with other actins and related proteins like actin-binding proteins makes it a critical factor in the molecular mechanisms underlying these diseases.