Abcam, ab12337, Anti-PIWIL1 antibody

Size: 100µg
Rabbit Polyclonal PIWIL1 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 46 publications.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PIWIL1 also known as Piwi-like protein 1 is a member of the Argonaute family with a molecular mass of approximately 97 kDa. This protein functions mechanically by binding to PIWI-interacting RNAs (piRNAs) which are small non-coding RNAs. PIWIL1 exhibits expression in germline cells specifically in testes therefore playing an important role in spermatogenesis. It facilitates the formation of piRNA complexes contributing to the regulation of gene silencing.
Biological function summary
PIWIL1 contributes to maintaining genomic integrity through its role in RNA-mediated gene regulation. It forms part of the piRNA-induced silencing complex. This complex targets transposable elements and prevents their mobility an important function for genomic stability. PIWIL1's involvement in gametogenesis highlights its essential function in reproductive biology and cellular processes where piRNA pathways operate.
Pathways
The PIWIL1 protein plays significant roles within the piRNA pathway which is important for transposon silencing and germline integrity. PIWIL1 interacts with MILI and MIWI two proteins that also participate in the silencing of transposable elements. This pathway intersects with other gene regulation pathways influencing the overall expression landscape necessary for proper cellular function in reproductive tissues.
PIWIL1 alterations have associations with germ cell tumors and fertility issues. Disruption in piRNA pathway components like PIWIL1 and its partners MIWI and MILI can lead to failures in genomic defense mechanisms influencing cancer development in germline cells. Additionally defects in PIWIL1 expression can contribute to infertility due to its role in spermatogenesis. Understanding these associations provides insights into potential diagnostic and therapeutic targets for related conditions.