Abcam, ab124465, Anti-ASS1 antibody [2B10]

Size: 100µL
Anti-ASS1 antibody [2B10] (ab124465) is a mouse monoclonal antibody detecting ASS1 in Western Blot, Flow Cytometry, ICC/IF, ELISA . Suitable for African green monkey, Human, Mouse, Rat . - KO validated for confirmed specificity - Over 10 publications
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:2B10,
Isotype:IgG1,
Carrier free:No,
Reacts with:Mouse, Rat, Human, African green monkey,
Applications:ICC/IF, ELISA, WB, Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human ASS1. The exact immunogen used to generate this antibody is proprietary information.P00966

Product details:
What is this antibody validated in?
Anti-ASS1 antibody [2B10] (ab124465) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA in African green monkey, Human, Mouse, Rat samples.
What is the molecular weight of ASS1?
Anti-ASS1 [2B10] (ab124465) specifically detects a band for ASS1 (UniProt: P00966) at a molecular weight of 47kDa.
Trusted by the scientific community
Anti-ASS1 [2B10] (ab124465) was first used in a scientific publication in 2012 and has been cited over 10 times in peer-reviewed journals.
Specificity confirmed
The specificity of Anti-ASS1 antibody [2B10] (ab124465) has been confirmed by Western blot testing in ASS1 Knockout HAP1 cell line,
ab264989

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Purified from tissue culture supernatant., Storage buffer-Preservative: 0.05% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ASS1 or argininosuccinate synthase 1 is an enzyme that plays an important role in the urea cycle. It catalyzes the conversion of citrulline and aspartate into argininosuccinate using ATP in the process. The ASS1 protein is commonly expressed in the liver where it actively participates in the detoxification of ammonia. It has a molecular mass of approximately 46 kDa. Some alternate names for this protein include ASS and ASS-1.
Biological function summary
Argininosuccinate synthase 1 contributes significantly to the synthesis of arginine an essential amino acid through its conversion activities. The enzyme operates as a homotrimer a complex consisting of three identical subunits. This enzymatic activity is indispensable in maintaining the balance of nitrogen and ammonia within the body particularly important for liver and kidney functions.
Pathways
ASS1 is a critical component of both the urea cycle and the nitric oxide metabolism pathway. The urea cycle helps in detoxifying ammonia by converting it into urea which is excreted in urine. In nitric oxide metabolism ASS1 links with nitric oxide synthase to facilitate the use of arginine in producing nitric oxide a vital signaling molecule. The pathway engagement partners include proteins like citrin which supplies aspartate for the reaction.
ASS1 has implications in citrullinemia and hepatocellular carcinoma. Citrullinemia results from mutations in the ASS1 gene leading to defective urea cycle function and ammonia accumulation. In cancer particularly hepatocellular carcinoma low expression of ASS1 correlates with poor prognosis as cancer cells often exhibit arginine auxotrophy. Connections through these conditions involve interactions with enzymes such as arginase which also influences arginine availability.