Abcam, ab124558, Recombinant Human LSM3 protein

Size: 100µg
Recombinant Human LSM3 protein is a Human Full Length protein, in the 1 to 102 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.
Key facts
Purity:>90% SDS-PAGE,
Expression system:Escherichia coli,
Tags:His tag N-Terminus,
Applications:Mass Spec, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:P62310,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.03% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
LSM3 also known as U6 snRNA-associated Sm-like protein LSm3 is a core component of the SMN-Sm complex. It has a molecular mass of about 10 kDa. LSM3 shows expression across various tissues with notable levels in the cytoplasm and nucleus. Its structure includes multiple Sm motifs responsible for RNA binding essential for its role in snRNP assembly.
Biological function summary
LSM3 works in forming part of the heptameric LSM protein complex. This complex plays an important role in RNA processing particularly in the stabilization and degradation of mRNA. LSM3 aids the functioning of U6 snRNA which is involved in pre-mRNA splicing a vital step in gene expression. It actively participates in the assembly and disassembly of pre-mRNA splicing machinery influencing mRNA turnover.
Pathways
LSM3 interacts significantly with spliceosomal pathways. It cooperates with proteins such as LSM2 and LSM4 facilitating the U6 snRNP assembly. LSM3 is integral to the mRNA decay pathway influencing how mRNAs are processed or degraded after undergoing splicing. Its interactions within these pathways highlight its role in post-transcriptional regulation critical for gene expression control.
LSM3 has links to neurodegenerative diseases like spinal muscular atrophy. In this disorder LSM3’s interaction with the SMN protein responsible for snRNP assembly becomes compromised affecting motor neurons. Defects in the SMN complex can lead to improper gene splicing and expression contributing to disease pathology. Additionally LSM3 links to certain cancers due to its role in the regulation of mRNA splicing and stability impacting cell proliferation and survival.