Abcam, ab125626, Recombinant human ARK5 protein

Size: 5µg / 10µg
Recombinant human ARK5 protein is a Human Full Length protein, in the 1 to 661 aa range, expressed in Baculovirus infected Sf9 cells, with >80%, suitable for SDS-PAGE, WB, FuncS.
Key facts
Purity:>80% Densitometry,
Expression system:Baculovirus infected Sf9 cells,
Tags:Tag free,
Applications:FuncS, WB, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:The specific activity of ab125626 was determined to be 50 nmol/min/mg.,
Accession:O60285,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 7.5Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

Product details:
ab204854
(Cdc25C peptide) can be utilized as a substrate for assessing kinase activity

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ARK5 also known as NUAK2 is a protein kinase that supports various cellular processes. It weighs approximately 72 kDa and belongs to the AMP-activated protein kinase (AMPK)-related family. ARK5 shows high expression in the brain muscle and liver tissues. Its kinase activity regulates the phosphorylation of target proteins influencing cell survival and metabolism in different environments.
Biological function summary
ARK5 plays a role in aiding cellular adaptation to stress. It participates in a complex network involving energy sensing and signal transduction. As a serine/threonine kinase it influences processes like cell proliferation and glucose metabolism by interacting with other signaling molecules. ARK5 activation contributes to the regulation of cellular energy homeostasis ensuring cells can respond to energy deficiencies effectively.
Pathways
ARK5 integrates with key cellular pathways like the PI3K/AKT/mTOR pathway. This protein kinase activates in response to external signals like growth factors and cellular stress linking to AKT to modulate downstream effects. ARK5 also interacts with LKB1 a tumor suppressor that phosphorylates AMPK creating a connection between metabolic regulation and cell growth signals impacting cellular energy regulation and stress responses.
ARK5 involvement is noted in cancer and metabolic syndromes. Elevated ARK5 activity associates with tumor progression and metastasis particularly in breast cancer where it may correlate with poor prognosis. Additionally its role in glucose metabolism suggests a link to type 2 diabetes where dysregulated pathways including ARK5 influence insulin sensitivity. This connection often includes proteins like AKT which also participates in these disorders' pathophysiology.