Abcam, ab125964, Human Complement C7 ELISA Kit

Size: 1 x 96Tests
Human Complement C7 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Complement C7 in Human in Biofluids samples.
Key facts
Detection method:Colorimetric,
Sample types:Cerebral Spinal Fluid, Saliva, Urine, Plasma, Milk, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.18 ng/mL,
Range:0.313 - 20 ng/mL,
Assay time:4h,
Assay Platform:Microplate

Product details:
Abcam's Complement C7 Human
in vitro
ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of C7 in Human plasma, serum, urine, milk, saliva, and CSF samples.
A Complement C7 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Complement C7 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Complement C7 captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Complement component 7 (C7) with a molecular mass of approximately 93 kDa plays an essential mechanical role in the complement system a critical part of innate immunity. C7 is one of the late components of the complement cascade involved in the formation of the membrane attack complex (MAC). It is primarily expressed in the liver and found in the blood plasma. By associating with other components C5b and C6 C7 anchors into the lipid bilayer of target cells serving as a foothold for further MAC assembly.
Biological function summary
C7 functions as an important facilitator in the innate immune response being an integral part of the membrane attack complex (MAC). This complex forms transmembrane channels in the cell membrane of pathogens leading to their lysis and destruction. Through its interaction with complement components like C5b and C6 C7 enables the insertion and stabilization of the MAC. This process is important for the immune system’s ability to eliminate pathogens quickly and effectively protecting the host from microbial invaders.
Pathways
The complement system pathway is the primary biological pathway involving C7. Within this pathway C7 interacts closely with complement proteins C5 and C6 contributing to the terminal pathway that leads to pathogen lysis. Besides it is linked with the classical and alternative complement pathways both of which converge at the activation of C5. Through these interactions and processes C7 works synergistically with other complement proteins to execute robust immune defense mechanisms.
C7 deficiencies have been associated with an increased susceptibility to infections particularly by Neisseria species due to impaired MAC formation. This link highlights the importance of C7 for adequate host defense. Additionally overactivation of the complement system potentially involving C7 contributes to the pathology of autoimmune disorders like systemic lupus erythematosus. In such conditions dysregulation involving proteins like C5b and C6 can lead to tissue damage and inflammation further illustrating the importance of C7 in maintaining immune homeostasis.