Abcam, ab128553, Recombinant human PPM1G protein

Size: 5µg / 10µg
Recombinant human PPM1G protein is a Human Full Length protein, in the 1 to 546 aa range, expressed in Baculovirus infected Sf9 cells, with >95%, suitable for SDS-PAGE, WB, FuncS.
Key facts
Purity:>95% SDS-PAGE,
Expression system:Baculovirus infected Sf9 cells,
Tags:Tag free,
Applications:SDS-PAGE, FuncS, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:The specific activity of ab128553 was determined to be 2,400 nmol/min/mg,
Accession:O15355,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 7.5Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.02% PMSF, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PPM1G also known as protein phosphatase 1G is a member of the protein phosphatase 2C (PP2C) family. This enzyme has a molecular mass of approximately 58 kDa. It localizes mainly in the nucleus and is expressed in various tissues including the brain testis and skeletal muscle. PPM1G functions as a serine/threonine phosphatase removing phosphate groups from target proteins and playing a role in the regulation of cellular stress responses and cell cycle progression.
Biological function summary
PPM1G contributes to several cellular functions by interacting with different substrates. As a component of the cellular machinery it is involved in processes such as RNA processing and regulation of mitosis. The enzyme participates in dephosphorylation events critical for the assembly and disassembly of the spliceosome complex which is important for accurate mRNA splicing. PPM1G's activity helps maintain the proper function of these cellular processes ensuring the stability and output of gene expression.
Pathways
Several significant cellular activities are influenced by PPM1G's regulatory mechanisms. It is implicated in the mitogen-activated protein kinase (MAPK) signaling pathway and the cell cycle regulation pathway. These pathways are vital for controlling cell growth and division. In these contexts PPM1G interacts with various other proteins such as p53 and cyclin-dependent kinases (CDKs) linking it to cell cycle checkpoints and stress responses. These interactions allow PPM1G to regulate and coordinate cellular responses to external and internal signals.
PPM1G has connections with illnesses like cancer and neurodegenerative diseases. In cancer its role in cell cycle regulation can affect tumor growth and progression often depending on its interactions with proteins like CDK. In neurodegenerative disorders PPM1G might play a part due to its involvement in RNA processing and protein dephosphorylation processes which are critical for neuron function. Any dysregulation in these interactions may contribute to disease pathology making PPM1G a point of interest for understanding and potentially treating these conditions.