Abcam, ab128894, Anti-SPAK antibody [EPR6394]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal SPAK antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 6 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR6394,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
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contact us
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Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.05% Sodium azideConstituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SPAK also known as Ste20-related Proline-Alanine-rich Kinase is a serine/threonine protein kinase with a molecular mass of approximately 61 kDa. It plays a critical mechanical role in the regulation of ion transport and cell volume by phosphorylating a variety of ion transporters and channels. SPAK expresses in many tissues including the kidney brain and heart which highlights its multifaceted functions in different physiological contexts.
Biological function summary
SPAK integrates into cellular signaling processes by contributing to the regulation of sodium potassium and chloride transport. This kinase mainly interacts within complexes of kinase networks that include OSR1 and WNK kinases. SPAK has essential roles in mediating responses to osmotic stress and modulating ion homeostasis reflecting its participation in regulating blood pressure and neuronal excitability.
Pathways
SPAK serves a significant role in the WNK signaling pathway and the MAPK pathway. WNK kinases activate SPAK through phosphorylation allowing SPAK to modulate the activity of co-transporter proteins. This interaction is important for managing ion fluxes across membranes. Furthermore SPAK's interplay with the MAPK pathway implicates it in cellular stress responses and proliferation where it coordinates with players like p38 MAPK to influence cell survival.
Researchers have linked SPAK to conditions like hypertension and epilepsy. Its association with hypertension arises through its involvement in sodium balance where disrupted SPAK activity can lead to increased blood pressure. SPAK also connects with epilepsy via modulation of neuronal ion transport playing a part alongside other proteins such as OSR1 in maintaining neuronal excitability. Understanding SPAK's role helps in developing strategies for managing these conditions.