Abcam, ab131330, Anti-nSMase antibody [EPR6718]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal nSMase antibody. Suitable for IP, WB and reacts with Mouse, Rat, Human samples. Cited in 6 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR6718,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NSMase also known as neutral sphingomyelinase is an enzyme with a molecular mass typically ranging between 43 to 48 kDa. It plays an important role in the hydrolysis of sphingomyelin to generate ceramide and phosphocholine. nSMase is distributed widely in various tissues with higher expression levels observed in the brain liver and spleen. This enzyme exists in multiple isoforms each localized in different cellular compartments contributing to diverse cellular functions.
Biological function summary
Neutral sphingomyelinase influences several cellular processes by modulating ceramide levels. Ceramide a bioactive lipid serves as a secondary messenger in signal transduction pathways and mediates processes like apoptosis and cell differentiation. nSMase often functions as a monomer but it can also be part of larger complexes with other proteins to execute its roles in cellular signaling. Through its enzymatic activity nSMase impacts membrane structure and dynamics affecting overall cellular homeostasis.
Pathways
Neutral sphingomyelinase is involved in key signaling pathways like the MAPK and apoptosis pathways. In the MAPK pathway nSMase-derived ceramide acts as a signaling molecule that can activate kinases involved in stress responses. Additionally its role in apoptosis links it to various stress signals where it facilitates the programmed cell death process. Interaction with proteins such as caspase and protein kinase C highlights its integration into signaling networks illustrating its regulatory significance.
Altered nSMase activity associates with cancers and neurodegenerative diseases. In cancer changes in ceramide production by nSMase can impact cell proliferation and survival connecting it with oncogenic processes. In neurodegenerative disorders such as Alzheimer's disease dysregulated nSMase activity influences neuroinflammation and neuronal cell death. It's interaction with amyloid precursor protein in such conditions highlights the enzyme's potential as a therapeutic target for modulating disease progression.