Abcam, ab131438, Anti-ERK1 (phospho Y204) antibody

Size: 100µL
Rabbit Polyclonal ERK1 phospho Y204 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 35 publications. Immunogen corresponding to Synthetic Peptide within Human MAPK3 phospho Y204 aa 200-250 conjugated to Keyhole Limpet Haemocyanin.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human MAPK3 phospho Y204 aa 200-250 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.P27361

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab131438 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide., Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ERK1 also known as MAPK3 is an extracellular signal-regulated kinase involved in transmitting signals from the cell surface to the nucleus. This protein has a molecular mass of about 44 kDa. ERK1 expresses in various tissue types with higher expression in the brain heart and skeletal muscle. Researchers often study ERK1 in the context of its role in cellular signaling due to its involvement in critical regulatory functions.
Biological function summary
ERK1 plays a significant role in cell cycle regulation differentiation and proliferation. It forms part of the MAPK signaling cascade becoming activated through a phosphorylation event. In its activated form ERK1 translocates to the nucleus where it phosphorylates target substrates. ERK1 often functions in conjunction with its homolog ERK2 to mediate these cellular processes marking it as an essential player in growth factor signaling.
Pathways
ERK1 functions primarily within the MAPK/ERK signaling pathway a major conduit for transmitting proliferative signals from growth factor receptors. ERK1 interacts with proteins like MEK1/2 which phosphorylate and activate ERK1 in response to extracellular stimuli. Another critical pathway involving ERK1 is the Ras-Raf-MEK-ERK cascade which regulates various cellular outcomes. This connection to the Ras family highlights its importance in signal transduction and reinforces its position in critical cellular processes.
Aberrant activation of ERK1 connects to diseases such as cancer and cardiovascular disorders. In cancer the dysregulation of the MAPK/ERK pathway often through mutations affecting Ras or Raf proteins leads to uncontrolled cell proliferation. ERK1's involvement in cardiovascular diseases links to its role in hypertrophic signaling in cardiac cells where altered ERK1 activity can contribute to pathological cardiac remodeling. Understanding these interactions can aid in developing therapeutic strategies targeting the MAPK/ERK signaling pathway.