Abcam, ab1318, Anti-CD63 antibody [NK1/C3]

Size: 250µL / 500µL
Mouse Monoclonal CD63 antibody. Suitable for ICC/IF, IHC-P, Flow Cyt and reacts with Human samples. Cited in 30 publications.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:NK1/C3,
Isotype:IgG1,
Light chain type:kappa,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, Flow Cyt, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Specificity:This antibody is specific to a 53 kD protein, expressed on activated platelets. CD63 is a lysosomal membrane glycoprotein that is translocated to plasma membrane after platelet activation. It is also expressed on granulocytes, B cells and T cells.

Product details:
CD63 is expressed on activated platelets. CD63 is a lysosomal membrane glycoprotein that is translocated to plasma membrane after platelet activation. It is also present in monocytes and macrophages and is weakly expressed in granulocytes, B, and T cells. CD63 is identical to the melanoma-associated antigen ME491 and to the platelet antigen PTLGP40.

Properties and Storage Information:
Form-Liquid, Purity-IgG fraction, Storage buffer-pH: 7.3 - 7.5Preservative: 0.05% Sodium azideConstituents: 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CD63 is a protein commonly known as LAMP-3 a member of the tetraspanin family with a molecular weight of approximately 25-65 kDa depending on post-translational modifications such as glycosylation. It resides mainly on the membrane of intracellular vesicles such as lysosomes and platelets. CD63 also appears on the surface of activated cells particularly in immune cells. Researchers often use CD63 as a marker in assays including CD63 western blot techniques due to its distinct expression patterns.
Biological function summary
CD63 participates in various cellular processes including membrane trafficking cell adhesion and signal transduction. It associates with integrins and other tetraspanins forming complexes that influence cellular adhesion and migration. Through its role in exosome formation CD63 contributes significantly to intercellular communication by facilitating the transfer of proteins lipids and RNA between cells.
Pathways
CD63 plays a role in pathways linked to cell proliferation and migration and immune response regulation. Within the integrin-mediated signaling pathway CD63 interacts with proteins such as integrins to promote cell adhesion and migration. CD63 also links to vesicle trafficking pathways collaborating with proteins involved in exocytosis and endocytosis which affect cell membrane restructuring and intracellular transport.
CD63 shows connections with cancer and immune system disorders. Its role in promoting cell adhesion and migration links it with cancer metastasis including melanoma and breast cancer where CD63 expression levels often rise. Additionally CD63 associates with allergic reactions due to its involvement in histamine release where it impacts immune proteins like FcεRI which participate in allergy pathogenesis.