Abcam, ab13259, Anti-Synaptotagmin antibody [ASV30]

Size: 100µg
Mouse Monoclonal SYT2 antibody. Suitable for WB, ICC/IF, IP and reacts with Rat, Mouse samples. Cited in 31 publications. Immunogen corresponding to Full Length Protein corresponding to Rat Syt1.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:ASV30,
Isotype:IgG2a,
Carrier free:No,
Reacts with:Mouse, Rat,
Applications:ICC/IF, WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Full Length Protein corresponding to Rat Syt1. The exact immunogen used to generate this antibody is proprietary information.P21707,
Specificity:ab13259 is not specific to SYT2 but is a pan-synaptotagmin antibody. It does not distinguish between the Synaptotagmin family of proteins.

Product details:
This product was changed from ascites to tissue culture supernatant on 5th July 2019. Lot numbers higher than GR3258922 are from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Purified from TCS., Storage buffer-Preservative: 0.09% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Synaptotagmin acts as a critical calcium sensor in the process of neurotransmitter release within synaptic vesicles. Also known as Syt synaptotagmin exhibits a mass of approximately 60 kDa. It is primarily expressed in neuronal tissues as well as in vesicles associated with secretory pathways. Synaptotagmin functionality relies on its ability to bind calcium ions facilitating the rapid fusion of vesicles with the plasma membrane during neurotransmission.
Biological function summary
Synaptotagmins play a central role in synaptic transmission and vesicular trafficking. They belong to a family of proteins involved in exocytosis where they interact as part of the SNARE complex. Synaptotagmin acts as a pivotal component by triggering vesicle fusion when it detects an influx of calcium ions. This process ultimately leads to the release of neurotransmitters into the synaptic cleft impacting numerous neural communication mechanisms.
Pathways
Synaptotagmins contribute significantly to synaptic vesicle cycling and calcium-mediated neuronal transmission. This protein is part of the synaptic vesicle cycle influencing pathways that regulate neurotransmitter release in response to calcium influx. It associates with proteins such as SNAP-25 and syntaxin working together to support vesicle docking and fusion processes essential for efficient synaptic signal transmission.
Synaptotagmins have connections to conditions like schizophrenia and bipolar disorder due to their role in neurotransmitter release regulation. Aberrant function or expression of synaptotagmins may influence synaptic activity contributing to these neuropsychiatric disorders. In some cases synaptotagmin dysfunction alters interactions with other proteins like complexins which impacts synaptic vesicle exocytosis and may relate to neurological conditions.