Abcam, ab132787, Recombinant Human PNPLA3 protein

Size: 10µg
Recombinant Human PNPLA3 protein is a Human Full Length protein, in the 1 to 481 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.
Key facts
Expression system:Wheat germ,
Tags:GST tag N-Terminus,
Applications:SDS-PAGE, WB, ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:Q9NST1,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 0.79% Tris HCl, 0.31% Glutathione

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PNPLA3 also known as patatin-like phospholipase domain-containing protein 3 is an enzyme that catalyzes the hydrolysis of ester bonds in glycerophospholipids. This protein has a molecular mass of about 53 kDa and is mainly expressed in liver tissue as well as in adipose tissue. It plays a role in lipid remodeling and triglyceride metabolism. The gene for PNPLA3 is located on chromosome 22 and its expression levels can influence its function in cellular lipid dynamics.
Biological function summary
PNPLA3 impacts lipid metabolism and storage in the liver. It does not function as part of a complex but rather exerts its effects independently. PNPLA3 modifies lipid droplet contents by converting triglyceride substrates. This activity helps regulate lipolysis affecting lipid droplet size and number in liver cells. Its function ensures a balance in hepatic lipid levels which is important for maintaining proper liver function and overall metabolic health.
Pathways
PNPLA3 is integral to lipid metabolic pathways and energy homeostasis. It interacts with key players in triglyceride metabolism such as DGAT2 and PNPLA2. These proteins especially DGAT2 help in the transformation and storage of triglycerides reflecting PNPLA3's role in regulating lipid balance. PNPLA3's interactions and functions in these pathways highlight its part in energy storage and utilization processes.
PNPLA3 genetic variants associate with non-alcoholic fatty liver disease (NAFLD) and liver fibrosis. Its role in these conditions relates to its impact on liver triglyceride content. The I148M variant of PNPLA3 has a notable connection to an increased risk of developing NAFLD. Additionally PNPLA3 interacts with proteins involved in fibrogenesis pathways such as TGF-β which influences the progression of liver disease towards fibrosis. Understanding these interactions helps researchers develop targeted therapies for liver conditions.