Abcam, ab133031, 15-deoxy-delta12,14-PGJ2 ELISA Kit

Size: 1 x 96Tests
15-deoxy-delta12,14-PGJ2 ELISA Kit is a competitive ELISA designed to quantify 15-deoxy-delta12,14-PGJ2 with a sensitivity of 36.8 pg/mL - Colorimetric competitive ELISA - 450 nm readout - works on any plate reader - Wide dynamic range - quantifies 195 - 200000 pg/ml
Key facts
Detection method:Colorimetric,
Sample types:Saliva, Urine, Plasma, Cell culture supernatant,
Reacts with:Human, Pig,
Assay type:Competitive,
Sensitivity:= 36.8 pg/mL,
Range:195 - 200000 pg/mL,
Assay time:5h,
Assay Platform:Microplate

Product details:
Abcam's 15-deoxy-delta
in vitro
competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of 15-deoxy-delta
in tissue culture media, saliva, urine, and plasma from a wide range of species.
A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-15-deoxy-delta
antigen and a polyclonal rabbit antibody specific to 15-deoxy-delta
. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of 15-deoxy-delta
captured in the plate.
15-deoxy-Δ
is one of the ultimate dehydration products of PGD
is formed from PGH
which itself is synthesized from arachidonic acid by the enzyme prostaglandin synthase. In the aqueous solution PGD
forms PGJ
. Albumin or other serum proteins present in the sample will convert PGD
to the isomeric compound, Δ
. In males about 152 ng of Δ
is excreted in urine per day, and in women about half this amount is excreted. 15-deoxy-Δ
has been shown as an inducer of adipogenesis and an activator of the γ isoform of the peroxisome proliferation activated receptor (PPARγ).
Cross Reactivity
Compound
Cross Reactivity
15-deoxy-Δ
Arachidonic Acid
<0.01%
<0.01%
9α,11β-PGF
<0.01%
Thromboxane B
<0.01%
2-Arachidonoylglycerol
<0.01%
Anandamide
<0.01%

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
15-deoxy-Δ1214-prostaglandin J2 (15-deoxy-Δ1214-PGJ2) acts as a potent endogenous ligand for peroxisome proliferator-activated receptor gamma (PPARγ) a nuclear receptor. It is a derivative of prostaglandin D2 and its molecular mass is approximately 316.4 g/mol. This compound typically arises in various tissues during inflammation or oxidative stress situations where it executes its regulatory effects.
Biological function summary
Through binding to PPARγ 15-deoxy-Δ1214-PGJ2 plays a role in modulating gene expression involved in lipid metabolism and adipogenesis. Its interaction with PPARγ influences the expression of anti-inflammatory cytokines and influences the inflammatory response making it significant in controlling inflammation. This compound does not form part of a larger protein complex but acts as an important signaling molecule in cellular processes.
Pathways
Several signaling pathways are affected by 15-deoxy-Δ1214-PGJ2 especially the nuclear receptor signaling pathway including the PPAR pathway. This pathway impacts key metabolic processes and regulates genes like adipocyte fatty-acid-binding protein (aP2). Additionally it links with the NF-kappa B signaling pathway through its interaction with proteins like IκBα affecting the transcription of genes involved in immune responses.
Increased levels of 15-deoxy-Δ1214-PGJ2 appear related to metabolic disorders such as type 2 diabetes and cardiovascular diseases. PPARγ to which 15-deoxy-Δ1214-PGJ2 binds holds relevance for insulin sensitivity and glucose homeostasis in diabetes. Additionally in cardiovascular diseases its effects on inflammation and lipid metabolism become significant linking with proteins such as leptin highlighting its role in disease mechanisms.