Abcam, ab133034, 12(S)-HETE ELISA Kit

Size: 1 x 96Tests
12(S)-HETE ELISA Kit is a Competitive ELISA for the measurement of 12(S)-HETE in in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant,
Assay type:Competitive,
Sensitivity:= 146.3 pg/mL,
Range:195 - 50000 pg/mL,
Assay Platform:Microplate

Product details:
Abcam's 12(S)-HETE
in vitro
competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of 12(S)-HETE in cell culture supernatants and plasma (heparin, EDTA).
A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-12(S)-HETE antigen and a polyclonal rabbit antibody specific to 12(S)-HETE. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the alkaline phosphatase enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of 12(S)-HETE captured in the plate.
12(S)-HETE is the stereo specific hydroxy product from the reduction of 12(S)-hydroperoxy tetraenoic eicosatetraenoic acid [12(S)-HpETE], which itself is a 12-lipoxygenase metabolite of arachidonic acid. 12(S)-HETE has been shown to be chemotactic and chemokinetic for polymorphonuclear leukocytes and vascular smooth cells. It also acts as a second messenger in angiotensin-II induced aldosterone production. Evidence also suggests that 12(S)-HETE is involved in suppressing renin production, stimulating insulin secretion by pancreatic tissue, inducing endothelial cell retraction and tumor cell adhesion.
Cross Reactivity
Compound
% Cross Reactivity
12(S)-HETE
12(R)-HETE
15-HETE
5(S)-HETE
8,15-diHETE
5,15-diHETE
6-keto-PGF
Thromboxane B
Arachidonic Acid
Leukotriene B
Leukotriene C
Leukotriene D
Leukotriene E
8-HETE
9-HETE
11-HETE

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
12(S)-HETE also known as 12(S)-hydroxyeicosatetraenoic acid is a bioactive lipid with a molecular mass of approximately 320 g/mol. It derives from arachidonic acid through the action of the 12-lipoxygenase enzyme. Cells such as platelets leukocytes and many types of epithelial cells express 12(S)-HETE. It plays a role in signaling mechanisms that manage various cellular processes such as inflammation and vascular function. Its effective concentration and local action make it integral to specific tissue environments.
Biological function summary
12(S)-HETE modulates cell movement proliferation and survival. It often interacts with membrane receptors and influences intracellular signal transduction pathways. Although not typically part of a large protein complex it acts closely with cellular structures to modify calcium signaling and gene expression. It impacts processes that could lead to tissue remodeling or repair especially under conditions of inflammatory stress.
Pathways
The role of 12(S)-HETE becomes evident in arachidonate metabolism and MAPK signaling. The lipid mediator influences signaling networks that are critical for immune responses and cellular stress reactions. Within these pathways it interacts with proteins such as cyclooxygenases and leukotrienes. These interactions suggest its modulatory capacity in balancing anti-inflammatory and pro-inflammatory signals.
12(S)-HETE contributes significantly to pathologies like cardiovascular disease and cancer. It regulates endothelial function and vessel tone affecting conditions like atherosclerosis. Cancerous tissues often show altered levels of 12(S)-HETE indicating its role in tumor growth and metastasis. In these diseases it may engage with proteins like VEGF and MMPs to promote angiogenesis and tissue invasion. Understanding its involvement provides insights into potential therapeutic target opportunities.