Abcam, ab133035, 15(S)-HETE ELISA Kit

Size: 1 x 96Tests
15(S)-HETE ELISA Kit is a competitive ELISA designed to quantify 15(S)-HETE with a sensitivity of 69.21 pg/mL - Colorimetric competitive ELISA - 450 nm readout - works on any plate reader - Wide dynamic range - quantifies 78.1 - 20000 pg/ml - Cited in over 10 publications
Key facts
Detection method:Colorimetric,
Sample types:Urine, Plasma, Cell culture supernatant, Serum,
Assay type:Competitive,
Sensitivity:= 69.21 pg/mL,
Range:78.1 - 20000 pg/mL,
Assay Platform:Microplate

Product details:
Abcam's 15(S)-HETE
in vitro
competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of 15-hydroxyeicosatetraenoic acid in plasma, serum, urine, tissue culture media and other biological fluids.
A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-15(S)-HETE antigen and a polyclonal rabbit antibody specific to 15(S)-HETE. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of 15(S)-HETE captured in the plate.
15(S)-HETE (15-hydroxyeicosatetraenoic acid) is the major hydroxy derivative of arachidonic acid when acted upon by 15-lipoxygenase (15-LOX). It is also the primary monohydroxy acid synthesized by the lipoxygenase activity of Cyclooxygenase-1. Aspirin-mediated acetylation of the COX-1 enzyme results in 15(R)-HETE. Blood platelets, peripheral leukocytes, vascular smooth muscle and other cell types produce Type-1 15-LOX while prostate, lung, skin and cornea tissues produce Type-2. 15-HETE has been proposed to act as a paracrine regulator of smooth muscle and lung neutrophil recruitment due in part to its incorporation into tracheal epithelium at the sn-2 position of phosphatidylinositol. The phosphoinositol modification in turn is thought to affect signal transduction and the regulation of intracellular calcium. Interleukin-4 has been shown to regulate 15(S)-HETE expression and incorporation into cellular phospholipids. 15(S)-HETE binds to actin and the alpha-subunit of mitochondrial ATP synthase suggesting a more direct method in regulating some physiological activities. Increased levels of 15(S)-HETE are associated with asthma, rhinitis, chronic paranasal sinusitis and rheumatoid arthritis.
Cross Reactivity
Compound
% Cross Reactivity
15(S)-HETE
5,15-diHETE
8,15-diHETE
13(S)-HODE
5-HETE
12(S)-HETE
12(R)-HETE
Arachidonic Acid
Linoleic Acid

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
15(S)-HETE also known as 15(S)-hydroxyeicosatetraenoic acid is a bioactive lipid molecule with a molecular mass of approximately 320.5 g/mol. This lipid derives from arachidonic acid through the enzymatic action of lipoxygenases primarily 15-lipoxygenase (15-LOX). It is expressed in various tissues including the lung skin and inflammatory cells. Its production occurs predominantly in response to cellular conditions that activate lipoxygenase pathways contributing to its role in various bodily processes.
Biological function summary
15(S)-HETE functions as a signaling molecule involved in inflammation and cell growth regulation. It participates in modulating cellular responses including acting on endothelial cells where it influences vascular tone and permeability. 15(S)-HETE is not part of a larger protein complex but it interacts with receptors on the cell surface and intracellular signaling pathways. Its role in inflammatory responses and vascular homeostasis is critical for normal physiological processes.
Pathways
15(S)-HETE contributes significantly to the inflammation and arachidonic acid metabolism pathways. It is closely related to prostaglandin and leukotriene synthesis positioning it as an important player in the regulation of inflammation. Within these pathways it interacts with proteins such as cyclooxygenase enzymes and other lipoxygenase isozymes influencing their activities and the production of other eicosanoids that mediate inflammatory responses.
15(S)-HETE shows connections to asthma and atherosclerosis. In asthma it participates in the regulation of airway inflammation and bronchial reactivity often involving interactions with proteins such as cytokines and eosinophils that exacerbate the inflammatory response. For atherosclerosis it is implicated in plaque formation through its effects on endothelial cells and macrophages highlighting its role in modulating the chronic inflammatory environment associated with the disease.