Abcam, ab133046, Mouse IgG2a ELISA Kit

Size: 1 x 96Tests
Mouse IgG2a ELISA Kit is a sandwich ELISA designed to quantify Mouse IgG2a with a sensitivity of 47.3 pg/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Wide dynamic range - quantifies 7.81 - 250 ng/mL
Key facts
Detection method:Colorimetric,
Sample types:Serum, Tissue Culture Media,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:= 47.3 pg/mL,
Range:7.81 - 250 ng/mL,
Assay Platform:Microplate

Product details:
Mouse IgG2a ELISA Kit ab133046 is a sandwich ELISA to measure Mouse IgG2a in serum, tissue culture media with a sensitivity of 47.3 pg/ml.
How the assay works
specific antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and along with an HRP-conjugated IgG
detection antibody and the microplate is then incubated at room temperature. After the removal of unbound proteins by washing, TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a colored product that changes after adding acidic stop solution. The density of coloration is directly proportional to the IgG
amount of sample captured in plate.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Mouse IgG2a ELISA Kit ab133046 protocol summary
1. Add standard or sample to each well used. Incubate at room temperature.
2. Aspirate and wash each well.
3. Add prepared HRP labeled secondary detector antibody. Incubate at room temperature
4. Aspirate and wash each well.
5. Add TMB Substrate Solution to each well.
6. Immediately begin recording the color development
IgG2 is divided into two subclasses; IgG2a and IgG2b. It is a glycoprotein which consists of two identical heavy chains (50 kDa each) and two identical light chains (25 kDa each), to give a combined mass of approximately 150 kDa. The chains are held in place by covalent disulfide bonds. Each light chain contains two immunoglobulin (Ig) domains, while the heavy chains contain four Ig domains each. In the middle of each heavy chain is a relative varying portion called the "hinge region" which is unique to each IgG. This region allows for molecular flexibility and sets IgG2a apart from its IgG counterparts. Properties of IgG2a include neutralization of toxins and diffusion in the extracellular space.
Cross Reactivity
% Cross Reactivity
mouse IgG2a
mouse IgG3
mouse IgM
mouse IgG1
mouse IgG2b
rat IgG2a

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols