Abcam, ab133047, Mouse IgM ELISA Kit

Size: 1 x 96Tests
Mouse IgM ELISA Kit is a sandwich ELISA designed to quantify Mouse IgM with a sensitivity of 0.6 ng/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Wide dynamic range - quantifies 3.91 - 250 ng/mL - Cited in over 10 publications
Key facts
Detection method:Colorimetric,
Sample types:Cell culture supernatant, Serum, Ascites Fluid,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.6 ng/mL,
Range:3.91 - 250 ng/mL,
Assay time:90m,
Assay Platform:Microplate

Product details:
Mouse IgM ELISA Kit ab133047 is a sandwich ELISA to measure Mouse IgM in serum, cell culture supernatant, ascites fluid with a sensitivity of 0.6 ng/ml.
How the assay works
IgM specific antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and along with an HRP-conjugated IgM detection antibody and the microplate is then incubated at room temperature. After the removal of unbound proteins by washing, TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a colored product that changes after adding acidic stop solution. The density of coloration is directly proportional to the IgM amount of sample captured in plate.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Mouse IgM ELISA Kit ab133047 protocol summary
1. Add standard or sample to each well used. Incubate wells.
2. Aspirate and wash each well.
3. Add prepared HRP labeled secondary detector antibody. Incubate wells.
4. Aspirate and wash each well.
5. Add Chromogen Substrate Solution to each well. Immediately begin recording the color development.
IgM is one of the most primitive and least specialized immunoglobulins. It is one of the five classes of antibodies found in mammals and it contains μ class heavy chains. The half-life of IgM is 5 days and it has a high molecular weight of 900,000 Daltons, which prevents its passage into extravascular areas. IgM levels are dependent on the extent of antigenic stimulation from the environment3. A developing B cell always makes IgM before any other class of antibody. IgM formed in B cells is a four-chain molecule composed of two light chains and two heavy chains. Once IgM molecules are formed in B cells, they are inserted into the plasma membrane where they act as antigen receptors. IgM is also the major antibody class involved in a primary antibody response, during which IgM molecules are secreted into the blood in the form of a pentamer. In a pentamer, five four-chain IgM units are held together by disulfide bonds and a small glycopeptide known as a J (joining) chain, giving the molecule a total of 10 antigen binding sites.
Compound
Cross reactivity
mouse IgM
rat IgM
Human IgM
mouse IgG
mouse IgG
mouse IgG
mouse IgG

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
IgM also known as immunoglobulin M is a type of antibody with a molecular mass of approximately 970 kDa. It is the largest antibody present in the human circulatory system mostly found in plasma and lymphatic tissue. IgM is secreted primarily by B cells as a pentamer with a J chain providing a high avidity for antigens. When bound to an antigen IgM can activate the complement system a part of the immune system that helps clear pathogens from an organism.
Biological function summary
IgM acts as the first line of defense during the initial stages of an immune response. It plays an important part in agglutination and neutralization of pathogens forming a complex that alerts other components of the immune system to take action. IgM exists before class-switching to IgG providing immediate protection. It also plays a role in antibody-dependent cellular cytotoxicity. Although not typically a long-term solution its presence is critical for providing initial protection until more specific antibodies like IgG and IgA are produced.
Pathways
The immune response that involves IgM begins with the activation of the classical complement pathway. This pathway bridges the innate and adaptive immunity ensuring the communication and coordination of immune responses. The pathway involves C1q protein binding to IgM-antigen complexes. IgM also interacts with antigen-presenting cells facilitating the processing of antigens and interaction with T cells. These interactions connect IgM with other proteins like IgG and IgA influencing various immune response pathways.
IgM is associated with conditions such as Waldenström's macroglobulinemia and rheumatoid arthritis. Waldenström's macroglobulinemia involves the overproduction of IgM by clonal B cells leading to symptoms like anemia bleeding and neuropathy. Rheumatoid arthritis shows increased IgM levels as part of the autoimmune response affecting joints. In these diseases the IgM antibody interacts with rheumatoid factor and other immune proteins involved in inflammation demonstrating its significant role in autoimmunity and abnormal immune responses.