Abcam, ab133049, Rat Gastrin 1 ELISA Kit

Size: 1 x 96Tests
Rat Gastrin 1 ELISA Kit is a Competitive ELISA for the measurement of Rat Gastrin 1 in Rat in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum, Tissue Culture Media,
Reacts with:Rat,
Assay type:Competitive,
Sensitivity:= 78.1 pg/mL,
Range:78.1 - 5000 pg/mL,
Assay time:3h,
Assay Platform:Microplate

Product details:
Abcam's Gastrin 1 Rat
in vitro
competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Gastrin 1 in tissue.
A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-Gastrin I antigen and a polyclonal rabbit antibody specific to Gastrin I. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the coloration is inversely proportional to the amount of Gastrin I captured in the plate.
Gastrins are a family of sequence-related carboxyamidated peptides produced by endocrine G Cells of the antrum mucosa in response to a number of stimuli associated with digestion. Antral distension, partially digested proteins, amino acids, and vagal stimulation resulting from smelling, tasting, chewing or swallowing food all contribute to gastrin release from G Cell storage. In addition, caffeine, alcohol, hypoglycemia, antacids and elevated calcium levels will also stimulate gastrin release. Increased serum gastrin levels are associated with duodenal ulcers, Helicobacter pylori infections, colorectal carcinomas, and other tumors and cancerous lesions. Gastrin is the most potent stimulator of gastric acid secretion. Rat Gastrin I (G17) Pyr-Arg-Pro-Pro-Met-Glu-Glu-Glu-Glu-Glu-Ala-Tyr-Gly-Trp-Met-Asp-Phe-NH
Gastrin is synthesized as a 104 residue pre-pro-peptide on the rough endoplasmic reticulum, then post-translationally modified by cleavage and alpha-amidation to result in the active forms G34 and G17. Other forms also exist, but are not considered biologically significant. There are two types of G17 and G34, type II is sulfated at the tyrosine residue, while type I is not. Both G34 and G17 circulate and contribute to the stimulation of gastric acid secretion, but have different clearance rates. G34 is the major circulating Gastrin in fasting serum, but with G17, increases two to threefold after feeding until both are present in approximately equal amounts. Normal serum levels in rats have been reported over a range of 40-169 pg/mL with possible variations between animal sources.
Compound
Cross Reactivity (%)
Rat Gastrin
Cholecystokinin 26-33 (CCK-8)
Human Gastrin I
Mouse Gastrin I
Minigastrin (G13-I)
Gastrin Tetrapeptide (CCK-4)
Glucagon
Gastrin Inhibitory Polypeptide (GIP)
Bombesin
Somatostatin-14
Gastrin Releasing Peptide (GRP)

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Gastrin I commonly known simply as gastrin is a peptide hormone significant for stimulating gastric acid secretion. It consists of 17 amino acids with a molecular mass of about 2 kDa. Gastrin is produced mainly in the G cells of the stomach and duodenum. It often exists in several active forms collectively referred to as gastrins with the most active being gastrin I and gastrin II. The hormone also regulates the growth of the gastric mucosa and can be measured using gastrin ELISA kits for research or diagnostic purposes.
Biological function summary
Gastrin plays an essential role in the digestive system by modulating acid secretion in the stomach. It acts by binding to the cholecystokinin B (CCK-B) receptors on the gastric parietal cells. This binding stimulates the release of histamine from enterochromaffin-like cells which in turn activates gastric acid secretion. Gastrin does not function as part of a protein complex but interacts dynamically with other molecules to exert its effects on acid production and gastric cells proliferation.
Pathways
Gastrin significantly influences digestive and signaling pathways notably the gastrin-Cck-B receptor pathway and the MAPK/ERK pathway. Through these pathways gastrin regulates digestive enzyme secretion and cell growth. It works closely with histamine and somatostatin to maintain acid balance in the stomach.
An imbalance of gastrin levels implicates in certain gastrointestinal diseases such as Zollinger-Ellison syndrome and gastritis. Excessive gastrin can lead to hypergastrinemia causing ulcers and sometimes gastric cancer. This hormone's dysregulation interacts significantly with proteins involved in these conditions such as the CCK-B receptor highlighting its role in pathophysiology.