Abcam, ab133106, PPAR delta Transcription Factor Assay Kit

Size: 96Test
Abcam's PPAR delta Transcription Factor Assay Kit (ab133106) is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts.
Key facts
Detection method:Colorimetric,
Sample types:Nuclear Extracts, Suspension cells, Adherent cells,
Reacts with:Mouse, Human,
Assay type:Semi-quantitative,
Assay Platform:Microplate reader

Product details:
Abcam's PPAR delta Transcription Factor Assay Kit (ab133106) is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts.
A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) sequence containing the peroxisome proliferator response element (PPRE) is immobilized onto the bottom of wells of a 96-well plate. PPARs contained in a nuclear extract, bind specifically to the PPRE. PPAR delta is detected by addition of specific primary antibody directed against PPAR delta. A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm. ab133106 detects human PPAR delta.
This assay does not cross-react with PPAR gamma 1 and PPAR gamma 2 or PPAR alpha.
Peroxisome proliferator-activated receptors (PPARs) are ligand activated nuclear receptors. Three PPAR subtypes have been identified: alpha, delta and gamma. PPARs can be activated by polyunsaturated fatty acids, eicosanoids and various synthtic ligands.
PPAR delta is the least understood; however, identification of a high-affinity PPAR delta synthetic ligand suggests a potential role for this receptor in lipid metabolism and obesity. PPAR delta is ubiquitously expressed, but it is particularly abundant in tissues such as liver, intestine, kidney, abdominal adipose, and skeletal muscle.

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols