Abcam, ab133153, Anti-ZIC3 antibody

Size: 200µL
Goat Polyclonal ZIC3 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ZIC3 aa 150-200.
Key facts
Host species:Goat,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human ZIC3 aa 150-200. The exact immunogen used to generate this antibody is proprietary information.O60481,
Specificity:ab133153 is NOT expected to cross-react with other ZIC proteins.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide., Storage buffer-pH: 7.3Preservative: 0.02% Sodium azideConstituents: 99% Tris buffered saline, 0.5% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Zinc Finger Protein of the Cerebellum 3 (ZIC3) is a transcription factor involved in the regulation of gene expression. This protein weighing approximately 52 kDa contains a C2H2-type zinc finger domain that facilitates its binding to DNA. ZIC3 plays a role in the development of various body structures such as the heart and neural system. It has its expression mainly in embryonic tissue and neural crest cells. Additionally it appears in tissues like the prostate indicating its involvement beyond developmental stages.
Biological function summary
ZIC3 is essential for normal morphogenesis and is part of a class of proteins that contribute to the formation of body asymmetry during embryogenesis. By interacting with other proteins it acts as a sequence-specific DNA-binding transcription factor. ZIC3 regulates the activity of genes required for the growth and differentiation of cells in vital developmental processes. Though not a part of a larger protein complex it influences complex cellular interactions during embryonic patterning.
Pathways
ZIC3 functions primarily in the Nodal signaling pathway which plays a role in left-right asymmetry in embryos. Within this pathway ZIC3 works in tandem with proteins like Smad2 and Smad3 which transmit the signal for cell fate determination. ZIC3's influence extends to the Hedgehog signaling pathway impacting the regulation of target gene expression during development and interacting with proteins like GLI transcription factors which mediate the cellular response to Hedgehog signals.
ZIC3 mutations are associated with X-linked heterotaxy a congenital condition affecting the left-right asymmetry of thoracic and abdominal organs. It relates closely to proteins within the Nodal signaling pathway where disruptions can cause improper organ placement. Additionally ZIC3 mutations have implications in various congenital heart defects and interactions with other transcription factors such as PITX2 a downstream target of ZIC3 are significant in these cardiac conditions.