Abcam, ab133411, Low Fluorescence Western Membrane (PVDF)

Size: 10Membrane
Low Fluorescence Western Membrane - 0.45 µm (PVDF) (ab133411) are high quality, durable polyvinylidene difluoride (PVDF) pre-cut membranes (10 sheets) and suitable for immunoblotting applications. - Suitable for western transfer with fluorescent detection - Detection methos: Fluorescence, total protein stain. - High signal-to noise rations– with low background levels - Compatible with gels 7 x 9 cm and smaller
Key facts
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Form:SolidSee storage information

Product details:
Pre-cut membranes (10 sheets, 7 x 9 cm), compatible with gels 10 x 10 cm and smaller. Note: PVDF membranes must be activated prior to use. Membrane activation protocol: 1. Pre-wet the membrane in methanol for a few moments until the membrane is completely wet. 2. Transfer the wet membrane from methanol to purified water and incubate on a rocker or shaker for at least 5 minutes. Make sure that the membrane is fully immersed in water and does not float on the water's surface. 3. Transfer the membrane from water to transfer buffer, and incubate for 5 minutes.

Properties and Storage Information:
Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions-Ambient, Appropriate long-term storage conditions-Ambient