Abcam, ab133565, Anti-RBX1 antibody [EPR6850(B)]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal RBX1 antibody. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 20 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR6850(B),
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Mouse, Rat,
Applications:IP, WB, Flow Cyt (Intra), ICC/IF, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RBX1 also known as RING-box protein 1 or ROC1 acts as an E3 ubiquitin ligase part of the ubiquitin-proteasome system. This protein facilitates the transfer of ubiquitin from E2 enzymes to target substrates marking them for degradation. The molecular weight of RBX1 is approximately 12 kDa. RBX1 is mainly expressed in various human tissues with significant expression in the heart brain and skeletal muscle. Its expression in these tissues suggests a wide involvement in cellular processes.
Biological function summary
RBX1 associates with the cullin-RING ubiquitin ligase (CRL) complex. It interacts with cullin proteins and other regulatory subunits to promote the polyubiquitination and subsequent degradation of target proteins. This regulatory activity influences many cellular processes including cell cycle progression DNA repair and signal transduction. By ensuring the degradation of proteins at the right time RBX1 maintains cellular homeostasis.
Pathways
RBX1 plays an important role in the control of protein stability through the ubiquitin-proteasome pathway. This pathway is important for regulating key cellular functions like the entrainment of the cell cycle and apoptosis. RBX1 also interacts with proteins like SKP1 and CUL1 as part of the SCF complex which targets specific proteins for degradation. By modulating these pathways RBX1 contributes to the overall regulation of cellular growth and stress responses.
RBX1's malfunctioning links to various types of cancer and neurodegenerative diseases. In cancer altered RBX1 activity can lead to the inappropriate degradation of tumor suppressor proteins promoting uncontrolled cell division. RBX1 is also implicated in neurodegenerative disorders where misregulation of protein degradation contributes to the accumulation of damaged proteins exacerbating disease progression. Additionally the interaction of RBX1 with proteins like p53 in cancer and tau proteins in Alzheimer's illustrates its potential role in disease pathogenesis.