Abcam, ab133583, Anti-CENPE antibody [EPR4542(2)]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal CENPE antibody. Suitable for WB and reacts with Human samples. Cited in 13 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR4542(2),
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please
contact us
for more information.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CENPE also known as centromere-associated protein E or kinesin-like protein CENP-E plays a critical role in the alignment of chromosomes during mitosis. This protein with a molecular mass of approximately 312 kDa acts as a motor protein involved in chromosome movement. CENPE is mainly expressed in dividing cells and plays an important role during the metaphase stage of the cell cycle. The protein is localized at the kinetochores the protein complexes on chromatids where the spindle fibers attach during cell division.
Biological function summary
CENPE is a vital component for chromosome congression which ensures proper chromosome alignment and segregation. It is part of several essential complexes necessary for mitotic checkpoint signaling and the correction of chromosome-microtubule attachments. Without CENPE's function cells face increased risks of aneuploidy. The protein interacts with microtubules to drive the movement toward the center of the cell and is important in ensuring accurate cell division.
Pathways
CENPE is involved in the mitotic checkpoint and spindle assembly pathways. These pathways ensure the correct distribution of chromosomes during cell division and prevent genetic instability. CENPE interacts with other proteins such as BUBR1 and Mad2 which are integral parts of the mitotic checkpoint complex. Proper functioning of CENPE ensures the activation of these checkpoints preventing premature separation of chromatids and maintaining genomic integrity.
CENPE has links to various conditions characterized by chromosomal instability such as cancer. Aberrant expression or dysfunction of CENPE can lead to improper chromosome segregation contributing to aneuploidy a hallmark of many cancers. Moreover its function connects with other proteins like Aurora B kinase which also plays a role in chromosomal alignment and stability. Disruption in the functions of these proteins can promote tumor progression and malignancy.