Abcam, ab133989, Organelle Detection Western Blot Cocktail

Size: 200µL
Organelle Detection Western Blot Cocktail (ab133989) is part of the reagents, controls & accessories range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
Key facts
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Target:GAPDH di methyl K9See target data,
Reacts with:Mouse, Rat, Human,
Form:LiquidSee storage information,
Storage buffer:Preservative: 0.02% Sodium azideConstituents: 0.877% Sodium chloride, 0.357% HEPES

Product details:
ab133989 contains 4 mAbs each targeting a specific organelle marker. The presence of plasma membrane is determined by Anti-Sodium Potassium ATPase antibody; mitochondrion by Anti-ATP5A antibody; cytosol by Anti-GAPDH; and nucleus by Anti-Histone H3 (di methyl K9). This cocktail is suitable for determining the purity of organelle isolates prior to further characterization.
This product is particularly valuable to researchers working in organelle proteomics. Mass spectrometry is frequently used in this field to determine the protein content of targeted organelle isolates. These isolates are obtained using differential centrifugation, density gradient fractionation, biochemical enrichment, or affinity purification. Unfortunately, the various methods of purification available for organelle isolation are imperfect and leave behind contaminants from undesired regions of the cell. These contaminants are inevitable, but being aware of which contaminants are present is crucial for analysis of mass spectrometry results. The high sensitivity and species cross reactivity of the antibodies in this cocktail will quickly and easily reveal impurities caused by imperfect sample preparation.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Sodium Potassium ATPase also known as Na+/K+-ATPase is an essential membrane enzyme responsible for maintaining the electrochemical gradients of sodium and potassium ions across the plasma membrane. This enzyme has a known molecular mass of approximately 112 kDa. It is widely expressed in most animal cells especially in excitable cells such as neurons and muscle cells. Additionally Na+/K+-ATPase plays an important role in organelle function contributing to processes like nutrient uptake and waste removal.
Biological function summary
The Na+/K+-ATPase enzyme helps regulate cellular volume and influence cell cycle progression. It forms part of a larger protein complex and is vital for maintaining cellular homeostasis. Beyond its mechanical ion-pumping function it is involved in signaling pathways that impact cell growth and differentiation. Its activity is important for neurotransmission and muscle contraction influencing organelle isolation processes. Na+/K+-ATPase also interacts with other proteins like ATPase inhibitory factor 1 which modulates its function.
Pathways
Na+/K+-ATPase participates actively in ion transport pathways and signaling cascades. For instance it is important in the ATP hydrolysis-driven transport process which relates directly with the maintenance of membrane potential and cellular excitability. It interacts with the Wnt signaling pathway influencing cellular communication and tissue patterning. Proteins such as phospholemman and Src kinase have functional interactions with Na+/K+-ATPase modulating its activity and influence across these pathways.
Na+/K+-ATPase dysregulation associates with conditions such as hypertension and heart failure. Abnormal function of this enzyme can disrupt ion homeostasis leading to elevated blood pressure or cardiac dysfunction. It also links to neurological disorders like schizophrenia where altered Na+/K+-ATPase activity may affect neurotransmission. The enzyme's interaction with proteins like alpha-synuclein and amyloid-beta plays a role in these pathologies further impacting disease progression and symptomatology.