Abcam, ab134117, Anti-DENN antibody [EPR4919]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal DENN antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 6 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR4919,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.05% Sodium azideConstituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The DENN domain-containing proteins also referred to as DENN serve as specific guanine nucleotide exchange factors (GEFs) that activate Rab GTPases important for vesicular trafficking. These proteins generally weigh between 110 to 150 kDa. DENN proteins predominantly are found within cellular membranes including endosomes. This positioning is consistent with their involvement in vesicle trafficking and membrane dynamics.
Biological function summary
DENN domain proteins facilitate critical processes such as endocytic recycling autophagy and transport processes within cells. These proteins belong to a complex containing multiple subunits that coordinate the activation of Rab GTPases. Through these activities DENN proteins ensure proper membrane trafficking dynamics and cellular organization integral to maintaining cell function.
Pathways
DENN proteins participate in cellular pathways like endocytic recycling and autophagy. In the endocytic pathway DENN proteins regulate Rab GTPases such as Rab5 and Rab7 controlling vesicle formation and movement. They also play a part in mTOR signaling influencing cell growth and autophagy regulation. By interacting with these key pathways DENN domain proteins help to integrate signals and coordinate cellular responses to environmental changes.
Alterations in DENN proteins often relate to neurodegenerative disorders and cancer. Mutations in the DENN domain have links to conditions such as Parkinson’s disease where Rab GTPases affected are implicated in neuronal function and survival. In cancer dysregulation of DENN protein function can influence cellular proliferation and apoptosis affecting tumor development. DENN proteins through their interactions and related pathways represent significant factors in understanding and potentially addressing these conditions.