Abcam, ab134189, Anti-MHC class I + HLA A + HLA B antibody [EPR1394Y]

Size: 100µL / 1mL
Anti-MHC class I + HLA A + HLA B antibody [EPR1394Y] (ab134189) is a rabbit monoclonal antibody detecting HLA A in Western Blot, Flow Cytometry, IHC-P, ICC/IF . Suitable for Human . - Biophysical QC for unrivalled batch-batch consistency - Over 20 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR1394Y,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, IHC-P, Flow Cyt, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:In our hands this antibody did not work in WB with mouse samples, but customer data suggest that reactivity in ICC/IF and IHC. The target shows high sequence homology with HLA-C and HLA-E, and it is possible that this antibody will detect these proteins too.

Product details:
What is this antibody validated in?
Anti-MHC class I + HLA A + HLA B antibody [EPR1394Y] (ab134189) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.
What is the molecular weight of HLA A?
Anti-MHC class I + HLA A + HLA B [EPR1394Y] (ab134189) specifically detects a band for HLA A (UniProt: P04439) at a molecular weight of 41kDa.
Trusted by the scientific community
Anti-MHC class I + HLA A + HLA B [EPR1394Y] (ab134189) was first used in a scientific publication in 2012 and has been cited over 20 times in peer-reviewed journals.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.
Discover our selection of trial-size antibodies
Other related products
We have a range of other formats of antibody clone [EPR1394Y] also available for your convenience: ab134189, PE -
ab303107
, APC -
ab303108
, HRP -
ab303109
, Alkaline Phosphatase -
ab308909
, Alexa Fluor® 647 -
ab310194
, Alexa Fluor® 594 -
ab310635
, Alexa Fluor® 555 -
ab312162
, Alexa Fluor® 568 -
ab312649
, Alexa Fluor® 750 -
ab321088
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please
contact us
for more information.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MHC class I molecules including HLA-A and HLA-B are proteins integral to the immune system. They often refer to as HLA which stands for Human Leukocyte Antigens. Typically MHC class I proteins with a molecular mass of around 45 kDa are expressed on the surface of nearly all nucleated cells. Their main mechanical function is to present endogenous peptides to CD8+ cytotoxic T lymphocytes enabling the immune system to monitor and respond to intracellular changes such as infections or malignancies.
Biological function summary
These MHC molecules play a critical role in adaptive immunity. They form a complex with beta-2 microglobulin (Β2m) to achieve stability and function properly on cell surfaces. HLA-A and HLA-B bind to short peptides derived from intracellular proteins which are then transported through the endoplasmic reticulum. The peptide-MHC complex is then expressed on the cell surface where it facilitates recognition by T cell receptors guiding immune responses against infected or transformed cells.
Pathways
MHC class I molecules are deeply involved in the antigen processing and presentation pathway as well as the immune effector process. In the antigen processing pathway proteins within the cell undergo degradation by the proteasome creating peptide fragments. These fragments are transported into the endoplasmic reticulum by the TAP (transporter associated with antigen processing) proteins where they bind with MHC class I forming a stable complex. Proteins like TAP and proteasome subunits work closely with MHC class I to ensure proper immune surveillance.
Dysregulation or alterations in MHC class I molecules particularly HLA-A and HLA-B have been linked to autoimmune diseases and cancers. For instance specific HLA alleles associate with autoimmune conditions like Type 1 Diabetes where the immune system erroneously targets pancreatic cells. In cancers a downregulation of MHC class I molecules often occurs as a mechanism to evade immune detection. Altered expression of proteins like TAP can also disrupt antigen presentation contributing to the disease onset and progression.