Abcam, ab135248, Anti-RUNX3 antibody [2B3]

Size: 100µL
Mouse Monoclonal RUNX3 antibody. Suitable for Flow Cyt, WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 20 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RUNX3 aa 150-300.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:2B3,
Isotype:IgG2b,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:IHC-P, ICC/IF, WB, Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human RUNX3 aa 150-300. The exact immunogen used to generate this antibody is proprietary information.Q13761

Product details:
This product was changed from ascites to supernatant. Lot no's high than GR206339-20 are from Tissue Culture Supernatant

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Purified from tissue culture supernatant., Storage buffer-Preservative: 0.05% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RUNX3 also known as Runt-related transcription factor 3 is a protein that plays an important role in regulating gene expression. The protein has a mass of approximately 44 kDa. RUNX3 is found mainly in tissues like the gastrointestinal tract and neural systems. It functions as a DNA-binding transcription factor that regulates various genes responsible for important cellular processes.
Biological function summary
The function of RUNX3 involves the regulation of cell proliferation differentiation and apoptosis. RUNX3 often forms a complex with the core-binding factor beta (CBFβ) enhancing its stability and DNA-binding capability. This protein is essential in maintaining cellular homeostasis and influencing the immune response particularly through its role in T cell development.
Pathways
RUNX3 is heavily involved in the TGF-beta signaling pathway and the Wnt signaling pathway. It interacts with several proteins such as SMADs in the TGF-beta pathway to modulate gene expression that affects cell growth and survival. Interaction with beta-catenin in the Wnt signaling pathway further emphasizes its importance in regulating cell fate decisions.
Alterations in RUNX3 expression or function are linked to cancer including gastric cancer and colorectal cancer. It often shows reduced activity or expression in these cancers which associates with proteins like TGF-beta leading to impaired cell growth control. RUNX3 dysregulation is also connected to neurological disorders due to its role in neural development and function.