Abcam, ab136649, Anti-Nucleolin antibody [364-5]

Size: 100µg
Anti-Nucleolin antibody [364-5] (ab136649) is a mouse monoclonal antibody detecting Nucleolin in Western Blot, Flow Cytometry, IP, IHC-P, ICC/IF . Suitable for Human . - Over 10 publications
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:364-5,
Isotype:IgG1,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, Flow Cyt, IHC-P, IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.

Product details:
What is this antibody validated in?
Anti-Nucleolin antibody [364-5] (ab136649) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.
What is the molecular weight of Nucleolin?
Anti-Nucleolin [364-5] (ab136649) specifically detects a band for Nucleolin (UniProt: P19338) at a molecular weight of 76kDa.
Trusted by the scientific community
Anti-Nucleolin [364-5] (ab136649) was first used in a scientific publication in 2012 and has been cited over 10 times in peer-reviewed journals.
Other related products
We have a range of other formats of antibody clone [364-5] also available for your convenience: ab136649, Alexa Fluor® 488 -
ab154028
, Alexa Fluor® 647 -
ab198580
, Carrier free -
ab212672
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Properties and Storage Information:
Form-Liquid, Purification notes-ab136649 is purified by biochemical fractionation. Near homogeneity as judged by SDS-PAGE., Storage buffer-pH: 7.3Preservative: 0.02% Sodium azideConstituents: 0.88% Sodium chloride, 0.36% HEPES, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Nucleolin also known as C23 is a multifunctional protein mainly found in the nucleolus with a molecular weight of approximately 100 kDa. It plays a mechanical role in ribosomal RNA (rRNA) synthesis and ribosome biogenesis. Nucleolin is widely expressed in proliferating cells including HeLa culture and shows higher expression in cancer cells compared to normal cells. The nucleolin protein is involved in various nucleolar processes like the modulation of chromatin structure and nucleocytoplasmic transport.
Biological function summary
The nucleolin molecular weight protein contributes to regulating cellular processes such as chromatin remodeling rRNA transcription and mRNA stability. Nucleolin often forms complexes with various other proteins and nucleic acids enhancing its functionality within the cell nucleus. Its participation in processes like DNA replication and cell proliferation shows the central role nucleolin plays at the cellular level. It influences cellular responses by binding directly to specific RNA or DNA sequences.
Pathways
The nucleolin protein engages in major cellular pathways such as the RNA processing and p53 signaling pathways. Through the RNA processing pathway it interacts with proteins like fibrillarin to assist in rRNA maturation. Moreover within the p53 signaling pathway nucleolin regulates the activity of the tumor suppressor protein p53 impacting cell cycle progression and apoptosis. These interactions highlight nucleolin's role in maintaining cellular homeostasis and response to stress.
The nucleolin protein often shows altered expression in cancers and cardiovascular diseases. In cancer nucleolin's high expression levels correlate with tumor progression and metastasis making it a potential target for cancer therapies. In cardiovascular diseases nucleolin interacts with proteins like VEGF to influence angiogenesis which affects disease progression. Understanding nucleolin's involvement in these diseases provides insight into potential therapeutic targets and interventions.