Abcam, ab136721, Rabbit Anti-Sheep IgG H&L (Glucose Oxidase)

Size: 500µL
Suitable for IHC-Fr, IHC-P, ICC, ELISA, WB.
Key facts
Host species:Rabbit,
Target species:Sheep,
Target isotype:IgG,
Target specificity:Heavy & Light chains,
Pre-adsorbed:No,
Conjugation:Glucose Oxidase,
Applications:IHC-P, WB, IHC-Fr, ICC, ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Clonality:Polyclonal,
Isotype:IgG

Product details:
Do not store in plastic tubes (i.e. eppendorf) to avoid losing activity.
Preparation of Substrate Solutions:
ELISA: 10mg/ml D(+)glucose, 0.1mg/ml grade II (Rz=0.6) peroxidase, 0.35mg/ml OPD in 0.1M pH 5 citrate-phosphate buffer. Incubation 30 minutes at 37°C. Stop the reaction with V/2 volume of 2N H
. Reading at 492 nm.
Immunocytochemistry: 15mg/ml D(+)glucose, 0.5mg/ml blue thiazolyl, 0.35mg/ml phenazine methosulfonate to be dissolved in that order in a 0.1M pH 6.8 phosphate buffer. Incubation 20 to 30 minutes in darkness.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab136721 is purified by a very stringent purification protocol where non-specific and specific purifications alternate (affinity chromatography)., Storage buffer-Constituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Avoid freeze / thaw cycle