Abcam, ab136810, Cell Cycle (pCdk/pHH3/Actin) WB Cocktail

Size: 200µL
Cell Cycle (pCdk/pHH3/Actin) WB Cocktail (ab136810) is part of the reagents, controls & accessories range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
Key facts
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Reacts with:Mouse, Human,
Form:LiquidSee storage information,
Storage buffer:Preservative: 0.01% Sodium azideConstituents: 40% Glycerol (glycerin, glycerine), 0.7% Sodium chloride, 0.61% Tris, 0.05% BSA

Product details:
The Cell Cycle western blot cocktail (ab136810) is designed to provide a rapid assessment of cell cycle distribution based on molecular markers for the G1/S and M phases of the cell cycle. This is a mixture of three specific rabbit monoclonal primary antibodies targeting phospho-cdk2 Tyr15, phospho Histone H3 Ser10 and beta-actin. Cyclin-dependent kinase 2 (Cdk2) is maintained in an inactive state in G1/S by inhibitory phosphorylation on Tyr15. Histone H3 is phosphorylated at Ser10 when chromosomes condense during mitosis. Hence, elevated phospho-cdk2 Tyr15 indicates G1/S arrested cells and elevated phospho Histone H3 Ser10 indicates M-phase arrested cells when compared to asynchronous cycling control cells. An anti- Actin antibody is included as a loading control. These three readouts are easily resolved by western blot given their different molecular weights.
The recommended dilution for this cocktail is 1:250.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C