Abcam, ab137042, Anti-FECH antibody [EPR8312]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal FECH antibody. Suitable for WB and reacts with Human samples. Cited in 9 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR8312,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
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Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purity-Tissue culture supernatant, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
FECH also known as ferrochelatase is a mitochondrial enzyme responsible for catalyzing the final step of heme biosynthesis. It inserts ferrous iron into protoporphyrin IX to form heme an essential component of hemoglobin myoglobin and various cytochromes. FECH has a molecular weight of approximately 43 kDa and is expressed in tissues with high heme demand like bone marrow and liver. Alternative names for FECH include protoporphyrinogen IX ferrochelatase and protoporphyrin ferro-lyase.
Biological function summary
FECH plays an important role in the production of heme facilitating the incorporation of iron into protoporphyrin IX. This reaction is not operating alone; FECH functions as part of multienzyme complexes in the mitochondria tightly regulated to meet cellular heme requirements. The activity of FECH ensures the proper functioning of heme-dependent proteins impacting oxygen transport and cellular respiration.
Pathways
FECH significantly contributes to the heme biosynthesis pathway. It interacts closely with other enzymes in the pathway such as aminolevulinate synthase (ALAS) and porphobilinogen deaminase essential for the step-wise production of heme. FECH's interaction with these proteins ensures the efficient flow of intermediates throughout the heme biosynthesis pathway ultimately impacting the synthesis of important heme proteins like cytochromes.
FECH mutations lead to erythropoietic protoporphyria (EPP) a condition characterized by sensitivity to sunlight and liver complications. The defective FECH reduces heme production efficiency causing protoporphyrin accumulation in red blood cells. FECH also interacts with proteins involved in mitochondrial function affecting iron metabolism and potentially linking to disorders such as sideroblastic anemia where abnormal erythropoiesis occurs due to defects in iron incorporation.