Abcam, ab137550, Anti-Nrf2 antibody

Size: 50µL
Anti-Nrf2 antibody (ab137550) is a rabbit polyclonal antibody detecting Nrf2 in Western Blot, IP, ChIP . Suitable for Human . - KO validated for confirmed specificity - Over 400 publications
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, IP, ChIPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human NFE2L2 aa 100-450. The exact immunogen used to generate this antibody is proprietary information.Q16236

Product details:
Anti-Nrf2 antibody (ab137550) is a rabbit polyclonal antibody and is validated for use in ChIP, IP, WB in human, mouse, rat samples.
Anti-Nrf2 antibody (ab137550) has been cited over 408 times in peer reviewed journals and is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-Nrf2 antibody (ab137550) has high sensitivity and specificity.
The specificity of Anti-Nrf2 antibody (ab137550) has been confirmed by testing in knockout samples.
Anti-Nrf2 antibody (ab137550) has 7 independent reviews from customers.
Anti-Nrf2 antibody (ab137550) specifically detects Nrf2 (UniProt ID: Q16236; Molecular weight: 68kDa) and is sold in 50 µL selling sizes.
NRF2 (Nuclear Factor Erythroid 2-Related Factor 2) is a transcription factor that regulates the expression of antioxidant proteins that protect against oxidative damage triggered by injury and inflammation. It plays a crucial role in cellular defense mechanisms. Dysregulation of NRF2 has been implicated in various diseases, including neurodegenerative disorders like Alzheimer's (AD) and Parkinson's disease (PD), where oxidative stress and inflammation are key pathological features.
NRF2 is bound to ubiquitin when it is the nucleus so MW would be higher than cytoplasm.
Please note:
Nrf2 antibody (ab137550) detects no signal in most untreated samples for WB. Stimuli treated samples are recommended. Nrf2 expression is stimulated by oxidative stress, electrophiles and chemical activators (PMID: 25761198, PMID: 27638861 and PMID: 28587109).
WB protocol info included in legend for MDA-MB-231 nuclear extract treated with 30 μM tBHQ for 4 hours (+).
Abcam recommended secondaries - Goat Anti-Rabbit HRP (
ab205718
) and Goat Anti-Rabbit Alexa Fluor®488 (
ab150077
). Or search our wide range of secondary antibodies for use with your experiment.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7Preservative: 0.025% Proclin 300Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Nrf2 also known as nuclear factor erythroid 2-related factor 2 is a transcription factor with a molecular weight of approximately 66 kDa. It plays a mechanical role in regulating the expression of antioxidant proteins that protect against oxidative damage triggered by injury and inflammation. Nrf2 is ubiquitously expressed in various tissues including the liver lungs and skin. Nrf2 activation occurs when it translocates from the cytoplasm to the nucleus to bind the antioxidant response element (ARE) in the DNA starting transcription of target genes.
Biological function summary
Nrf2 acts as an important regulator of the cellular antioxidant response. It works in conjunction with its partner protein Keap1 forming a complex that controls its stability and degradation. Under normal conditions Keap1 keeps Nrf2 in the cytoplasm where it is targeted for ubiquitination and degradation. Once activated by oxidative stress or electrophiles Nrf2 dissociates from Keap1 thereby avoiding degradation and relocates to the nucleus to activate the transcription of ARE-dependent genes. This activity boosts the cellular response to oxidative stress by inducing genes involved in detoxification and cellular defense.
Pathways
Nrf2 plays a significant role in the oxidative stress response and detoxification pathways. Nrf2 activation is linked closely to the PI3K/Akt signaling pathway which influences cell survival growth and metabolism. This pathway also interacts with other important proteins like GSK-3β which can modulate Nrf2 activity and stability. Through these pathways Nrf2 orchestrates a defense mechanism against reactive oxygen species (ROS) by boosting the expression of antioxidant enzymes and detoxifying proteins.
Nrf2 has been associated with conditions like cancer and neurodegenerative diseases. In cancer aberrant Nrf2 activation may lead to enhanced tumor survival by increasing expression of cytoprotective genes which makes cancer cells resistant to chemotherapy. Nrf2 also interacts with proteins such as p53 which play roles in tumor suppression and cellular stress responses. In neurodegenerative disorders reduced Nrf2 activity can contribute to oxidative stress leading to neuron damage and disease progression with proteins like amyloid-beta also being linked with oxidative processes affected by Nrf2 functionality.