Abcam, ab137773, Anti-OGDH antibody - C-terminal

Size: 100µL
Rabbit Polyclonal OGDH antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 22 publications. Immunogen corresponding to Recombinant Fragment Protein within Human 2-oxoglutarate dehydrogenase, mitochondrial aa 700-1000.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:IHC-P, ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human 2-oxoglutarate dehydrogenase, mitochondrial aa 700-1000. The exact immunogen used to generate this antibody is proprietary information.Q02218

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7Preservative: 0.025% Proclin 300Constituents: PBS, 20% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
OGDH also known as alpha-ketoglutarate dehydrogenase or a-ketoglutarate dehydrogenase is an enzyme that plays a significant role in metabolism. It catalyzes the conversion of alpha-ketoglutarate into succinyl-CoA and CO2 in the tricarboxylic acid (TCA) cycle also known as the Krebs cycle. The enzyme functions as a part of a multi-enzyme complex and has a molecular mass of approximately 1 MDa. OGDH is expressed in various tissues with higher levels typically found in the mitochondria of cells reflecting its role in cellular respiration.
Biological function summary
OGDH is critical for energy production by enabling the TCA cycle to proceed. It is a component of the alpha-ketoglutarate dehydrogenase complex consisting of multiple subunits which include E1 (OGDH itself) E2 (dihydrolipoamide succinyltransferase) and E3 (dihydrolipoamide dehydrogenase). This complex functions to ensure the proper oxidation of metabolites important for maintaining cellular energy homeostasis. Proper regulation of OGDH activity impacts several metabolic pathways influencing cellular glucose and lipid metabolism.
Pathways
OGDH is integrally involved in the TCA cycle a central metabolic pathway that connects carbohydrate fat and protein metabolism. Alongside related enzymes like pyruvate dehydrogenase (PDH) and isocitrate dehydrogenase (IDH) OGDH contributes to the generation of NADH and FADH2 essential carriers in the electron transport chain. The interplay between these enzymes ensures the smooth functioning of aerobic respiration linking glycolysis and oxidative phosphorylation.
OGDH has links to neurodegenerative diseases such as Alzheimer's and Parkinson's disease. Abnormal activity of OGDH can lead to mitochondrial dysfunction a common feature observed in these disorders. The protein connects to pathways involving PDH dysfunction contributing to altered metabolic states seen in these conditions. Research continues to explore the potential of targeting OGDH in therapeutic strategies aimed at improving mitochondrial health and energy metabolism in affected patients.