Abcam, ab137872, Anti-LEF1 antibody [EPR2029Y]

Size: 20µL / 100µL / 1mL
Anti-LEF1 antibody [EPR2029Y] (ab137872) is a rabbit monoclonal antibody detecting LEF1 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF . Suitable for Human, Mouse, Rat . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 60 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR2029Y,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:IP, IHC-P, WB, ICC/IF, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Anti-LEF1 antibody [EPR2029Y] (ab137872) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-P, IP and WB.
Abcam's high quality manufacturing and validation processes ensure Anti-LEF1 antibody [EPR2029Y] (ab137872) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
The specificity of Anti-LEF1 antibody [EPR2029Y] (ab137872) has been confirmed by Western Blot testing in LEF1 knockout Jurkat cells (
ab274898
Anti-LEF1 antibody [EPR2029Y] (ab137872) specifically detects LEF1 (UniProt ID: Q9UJU2; Molecular weight: 45kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL).
Conjugation-ready, carrier free format available for antibody clone EPR2029Y -
ab215999
Antibody clone EPR2029Y is also available pre-conjugated to a variety of labels for your convenience - HRP, Alexa Fluor
647, APC (
ab197623
ab246715
ab314938
LEF1 is a transcription factor that promotes tumorigenicity in various cancers by activating pathways like TGF-β. LEF1 also plays a role in maintaining cancer stem cell properties, contributing to chemoresistance and metastasis.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
LEF1 (Lymphoid Enhancer-Binding Factor 1) also known as LEF-1 or LEF 1 functions as a transcription factor involved in Wnt signaling pathways. The LEF1 protein has a mass of about 45 kilodaltons. Expression of LEF1 occurs mainly in lymphoid tissues and the central nervous system. Researchers often use LEF1 in immunohistochemistry (LEF1 IHC) for its ability to bind DNA at specific sites regulating gene expression important for cellular development and differentiation especially in T cells such as Jurkat cells.
Biological function summary
LEF1 plays a significant role in the regulation of cell fate decisions and development. It acts as part of a transcription complex that binds to specific DNA sequences facilitating the transcription of target genes. LEF1 interacts with β-catenin and this interaction is essential for the regulation of gene expression during embryonic development and cellular proliferation. LEF1's biological functions make it a useful marker in various research applications and assays like LEF1 ELISA are often utilized to study its expression levels in different physiological contexts.
Pathways
LEF1 is a central player in the canonical Wnt/β-catenin signaling pathway. This pathway is fundamental for processes like embryogenesis and cellular growth. LEF1 interacts closely with proteins such as TCF (T-cell factor) family members and β-catenin to regulate gene expression. Disruption in the Wnt pathway where LEF1 is significant can lead to abnormal cell growth and differentiation. Consequently LEF1 serves as an indicator and potential regulator of pathway activities in various cellular environments.
LEF1 is associated with numerous conditions particularly in oncology and immunology. Aberrations in LEF1 expression contribute to diseases like leukemia and lymphoma. Overexpression or mutations of LEF1 might lead to unregulated Wnt signaling promoting cancer progression. Furthermore LEF1's interactions with proteins such as MYC a well-known oncogene highlight its importance in tumorigenesis. Understanding LEF1's mechanisms can offer insights into therapeutic targets for these diseases providing a pathway for research into drug development and intervention strategies.