Abcam, ab137969, Human Pancreatic Amylase ELISA Kit

Size: 1 x 96Tests
Human Pancreatic Amylase ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Pancreatic Amylase in Human in Biofluids samples.
Key facts
Detection method:Colorimetric,
Sample types:Saliva, Urine, Plasma, Milk, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:> 0.053 mIU/mL,
Range:0.156 - 10 mIU/mL,
Assay time:4h,
Assay Platform:Microplate

Product details:
Abcam's Pancreatic Amylase Human
in vitro
ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Human pancreatic amylase in plasma, serum, urine, milk, saliva samples.
A Pancreatic Amylase specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Pancreatic Amylase specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Pancreatic Amylase captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Pancreatic amylase also known as alpha-amylase or amylopsin is an enzyme with a molecular mass of approximately 55 kDa. This enzyme is expressed mainly in the pancreas and is secreted into the small intestine. Its primary mechanical function involves hydrolyzing starch into smaller carbohydrates such as maltose and dextrin. Pancreatic amylase activity is essential for the efficient digestion of dietary starch aiding in the breakdown into sugars that the body can easily absorb and utilize.
Biological function summary
The enzyme facilitates the digestive process by catalyzing the cleavage of 14-glycosidic bonds in polysaccharides. Pancreatic amylase operates independently and not as part of a larger protein complex. Its function exclusively converts complex carbohydrates into simpler absorbable sugars. This role is essential in human metabolism as it initiates the conversion of dietary carbohydrates into forms that the intestine can further process and transport throughout the body.
Pathways
Pancreatic amylase participates in the carbohydrate digestion and absorption pathway. It interacts with other enzymes such as maltase and isomaltase which further break down the products of its enzymatic activity. This enzyme plays a core role in the pathway ensuring proper starch conversion into glucose which cells can uptake for energy. Pancreatic amylase parallels salivary amylase which begins the starch digestion process in the mouth.
Pancreatic amylase levels serve as a marker for pancreatitis an inflammation of the pancreas. Elevated serum levels indicate pancreatic injury or inflammation pointing to disorders that affect enzyme secretion. Chronic pancreatitis or acute pancreatitis often correlates with increased pancreatic amylase. Furthermore in certain digestive disorders like cystic fibrosis the secretion of pancreatic amylase may be disrupted indicating its pivotal role in maintaining pancreatic function and health. The enzyme's activity measurement can aid in diagnosing these conditions and monitoring their progression.