Abcam, ab13876, Anti-TLR5 antibody [19D759.2]

Size: 100µg
Mouse Monoclonal TLR5 antibody. Suitable for ICC, IP, WB, IHC-P, ICC/IF, IHC-Fr, Flow Cyt (Intra) and reacts with Mouse, Human samples. Cited in 20 publications. Immunogen corresponding to Synthetic Peptide within Human TLR5 aa 700-800 conjugated to Keyhole Limpet Haemocyanin.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:19D759.2,
Isotype:IgG2a,
Light chain type:kappa,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:IHC-Fr, Flow Cyt (Intra), ICC/IF, IP, WB, ICC, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human TLR5 aa 700-800 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.O60602,
Specificity:This antibody recognizes an intracellular epitope in the cytoplasmic domain of TLR5.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Storage buffer-pH: 7.4Preservative: 0.05% Sodium azideConstituents: PBS, 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Toll-like receptor 5 (TLR5) also known as CD285 is a protein that functions mechanically as a pattern recognition receptor. It has a mass of about 98 kDa and is expressed mainly in the tissues of the lung intestine and urogenital tract. TLR5 recognizes pathogen-associated molecular patterns specifically identifying and binding to flagellin a protein component of bacterial flagella. This engagement initiates a signaling cascade that results in an immune response.
Biological function summary
This receptor plays a central role in the innate immune system. TLR5 is part of a complex network of receptors that detect microbial infections and activate immune defense. When TLR5 binds to flagellin it triggers the production of inflammatory cytokines and activation of adaptive immune responses. This interaction helps in mobilizing immune cells to the site of infection providing the body with a robust defense mechanism against bacterial pathogens.
Pathways
TLR5 activation is an important component of the NF-kB signaling and MAPK pathways. These pathways lead to the transcription of genes involved in inflammation and immune response. TLR5 interacts with MyD88 an adaptor protein to facilitate the downstream signaling cascade. This involvement links TLR5 to not only NF-kB pathway but also connects it to other Toll-like receptors further integrating the immune signaling network.
TLR5 has been connected to inflammatory bowel disease and chronic obstructive pulmonary disease (COPD). Abnormal TLR5 signaling can lead to excessive inflammation contributing to these conditions. In COPD TLR5 and its associated cytokines can exacerbate lung inflammation while in inflammatory bowel disease dysregulation may trigger improper intestinal immune responses. Research into TLR5's role in these diseases can illuminate potential therapeutic targets.