Abcam, ab13897, Anti-5HT3A receptor/5ht3 antibody

Size: 100µg
Rabbit Polyclonal 5HT3A receptor/5ht3 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 15 publications. Immunogen corresponding to Synthetic Peptide within Rat Htr3a.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Rat Htr3a. The exact immunogen used to generate this antibody is proprietary information.P35563

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The 5HT3A receptor also known as the 5-HT3 receptor or 5HT3 forms part of the serotonin receptor family. These ligand-gated ion channels enable the rapid conduction of cations such as Na+ and Ca2+ into cells upon activation. The 5-HT3A receptor exhibits a molecular weight of approximately 55 to 60 kDa and is expressed broadly in peripheral and central nervous systems including regions like the cortex hippocampus and gastrointestinal tract. This expression pattern allows it to perform roles in neurotransmission and modulate physiological responses across different systems.
Biological function summary
The 5-HT3A receptor acts as a critical mediator of synaptic signalling and neurotransmitter release offering a path for serotonin's effects. As part of a pentameric complex 5-HT3A subunits combine to form functional ion channels. The receptor particularly influences excitatory neurotransmission affecting processes involved in mood regulation nausea and vomiting. Consequently this receptor serves key physiological functions through its modulation of ionic flux and neuronal activity.
Pathways
The 5-HT3A receptor plays an essential role in pathways related to neurotransmission and neuroplasticity. Within serotonin signalling pathways 5HT3A closely associates with proteins such as serotonin transporter (SERT) elaborating on its role in serotonin uptake and homeostasis. Furthermore its participation in these pathways links to the regulation of calcium-dependent processes interacting with calmodulins and other calcium-binding proteins to influence downstream signalling events essential for cognitive processes and sensory signalling.
The 5-HT3A receptor has been associated with conditions like anxiety and irritable bowel syndrome (IBS). In anxiety disorders its role in modulating serotonin-induced neurotransmission points to its involvement in mood disorders where altered serotonin balance is a contributing factor. As for IBS the receptor's high expression in the gut highlights its influence on gastrointestinal motility and secretion. Researchers also explore its connection with GABAergic systems pointing to potential therapeutic targets for managing symptoms in these disorders.