Abcam, ab139188, Anti-NPR-A + NPR-B antibody [EPR5719(2)]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal NPR-A antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 4 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR5719(2),
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:The immunogen for this product shares 95% homology with NPR-A

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NPR-A and NPR-B are types of natriuretic peptide receptors which operate as transmembrane receptors composed of an extracellular ligand-binding domain a single transmembrane-spanning domain and an intracellular guanylyl cyclase catalytic domain. NPR-A is widely recognized as GC-A while NPR-B is also known as GC-B. These receptors are approximately 120 kDa in size. Both NPR-A and NPR-B are expressed in various tissues including the heart kidneys and vascular smooth muscle cells which suggests a broad physiological role across different body systems.
Biological function summary
These receptors function mainly by binding natriuretic peptides like atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP) for NPR-A while C-type natriuretic peptide (CNP) serves as the primary ligand for NPR-B. Their activation leads to the conversion of GTP to cGMP which then functions as a second messenger involved in vasodilation and diuresis. The receptors are essential components of blood pressure regulation and fluid balance through their role in the cGMP complex.
Pathways
For both NPR-A and NPR-B they significantly contribute to the maintenance of cardiovascular homeostasis and kidney function through the natriuretic peptide signaling pathway. They are tightly linked with proteins such as guanylyl cyclase-related proteins and phosphodiesterases which help in the regulation and degradation of cGMP respectively. Furthermore these receptors interact strongly with cyclic nucleotide signal transduction pathways that involve other key proteins including protein kinase G.
NPR-A and NPR-B hold importance due to their role in cardiovascular diseases including hypertension and heart failure. The dysregulation of these receptors alongside ANP and BNP has been associated with these conditions. Moreover they are implicated in skeletal dysplasia disorders due to the involvement of NPR-B in cartilage development where the signaling through NPR-B in chondrocytes plays a significant role. Malfunctions in this receptor system can lead to improper regulation of growth and development linking it with disorders such as achondroplasia alongside CNP.