Abcam, ab139415, Endoplasmic Reticulum Fraction Western Blot Cocktail

Size: 200µL
Endoplasmic Reticulum Fraction Western Blot Cocktail (ab139415) is part of the reagents, controls & accessories range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
Key facts
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Reacts with:Mouse, Rat, Human,
Form:LiquidSee storage information,
Storage buffer:Preservative: 0.0001% Sodium azideConstituents: 0.5% Glycerol (glycerin, glycerine), 0.009% Sodium chloride, 0.006% Tris, 0.0005% BSA

Product details:
ab139415 contains 3 mAbs each targeting a specific organelle marker. The presence of endoplasmic reticulum is detected by GRP78; cytosol by Anti-GAPDH; and nucleus by Anti-Histone H3 (di methyl K9). This cocktail is suitable for determining the purity of organelle isolates prior to further characterization.
This product is particularly valuable to researchers working in organelle proteomics. Mass spectrometry is frequently used in this field to determine the protein content of targeted organelle isolates. These isolates are obtained using differential centrifugation, density gradient fractionation, biochemical enrichment, or affinity purification. Unfortunately, the various methods of purification available for organelle isolation are imperfect and leave behind contaminants from undesired regions of the cell. These contaminants are inevitable, but being aware of which contaminants are present is crucial for analysis of mass spectrometry results. The high sensitivity and species cross reactivity of the antibodies in this cocktail will quickly and easily reveal impurities caused by imperfect sample preparation.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
GRP78 BiP also known as glucose-regulated protein 78 or heat shock protein A5 is a vital chaperone protein with a molecular mass of approximately 78 kDa. It is present in the endoplasmic reticulum where it assists in protein folding and quality control. GRP78 is widely expressed across various cell types and plays a significant role in managing stress within the endoplasmic reticulum. GAPDH or glyceraldehyde-3-phosphate dehydrogenase is an enzyme involved in glycolysis with a mass of around 36 kDa and it is pervasive in most tissues. Histone H3 a core component of the nucleosome weighs about 15 kDa and is essential for DNA packaging in eukaryotic cells.
Biological function summary
GRP78 BiP participates in maintaining cellular homeostasis by regulating the unfolded protein response and aiding in the assembly of multimeric protein complexes. GRP78 interacts closely with various other proteins to mitigate stress in the endoplasmic reticulum such as protein disulfide isomerase. GAPDH is central to energy production and intersects with various cellular processes including autophagy and nuclear functions. Histone H3 contributes to chromatin structure and gene expression functioning as part of the histone octamer complex.
Pathways
GRP78 BiP functions in the endoplasmic reticulum stress pathway connecting with key proteins like ATF6 during the unfolded protein response. GAPDH plays a role in the glycolytic pathway linking to enzymes such as hexokinase and pyruvate kinase facilitating energy production. Histone H3 is important in the DNA replication and repair pathway interacting with proteins such as histone acetyltransferases that modify chromatin structure and gene expression.
GRP78 BiP has associations with cancer and neurodegenerative disorders. It interacts with proteins like PERK in response to endoplasmic reticulum stress which contributes to tumor growth and progression. GAPDH relates to neurodegenerative diseases and its interaction with proteins such as S-nitrosylated caspase-3 can result in neuronal apoptosis. Histone H3 has a connection with cancer and is implicated in epigenetic changes associated with histone mutations and post-translational modifications impacting gene expression profiles in tumor development.