Abcam, ab140421, Recombinant Human UBA3 protein

Size: 20µg
Recombinant Human UBA3 protein is a Human Full Length protein, in the 1 to 463 aa range, expressed in Baculovirus infected Sf9 cells, with >95%, suitable for SDS-PAGE, WB.
Key facts
Purity:>95% Densitometry,
Expression system:Baculovirus infected Sf9 cells,
Tags:Tag free,
Applications:WB, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:Q8TBC4,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 7.5Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

Product details:
This product was previously labelled as UBE1C

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The target UBA3 also known as ubiquitin-like modifier-activating enzyme 3 functions mechanically as an essential enzyme in the NEDD8-conjugation pathway. It weighs approximately 50 kDa. UBA3 expresses itself in various tissues but shows widespread expression across different cell types. It operates as a catalytic subunit of the E1 enzyme complex that initiates the activation and transfer of the NEDD8 protein. This process is important for the subsequent conjugation to target proteins.
Biological function summary
The process involving UBA3 relates to protein modification primarily through neddylation. UBA3 couples with the protein NAE1 to form the NEDD8 activating enzyme complex. This enzyme complex activates NEDD8 a ubiquitin-like protein that modifies cullin-based E3 ligases influencing their function. By modifying these E3 ligases UBA3 controls various cellular processes including cell cycle progression and signal transduction ensuring proper cell function and adaptation to environmental cues.
Pathways
UBA3 is integral to the NEDD8 conjugation pathway influencing cell cycle and transcriptional regulation. It interacts with proteins such as cullin 1 and cullin 3 within this pathway. By activating NEDD8 UBA3 facilitates cullin-RING ubiquitin ligase complexes which play significant roles in the ubiquitin-proteasome pathway. This interaction controls protein degradation a vital process for maintaining protein homeostasis and preventing cellular dysfunction.
UBA3 involvement in neddylation links it to cancer and neurodegenerative diseases. Aberrant UBA3 activity relates to cancers as dysregulation of the NEDD8 pathway can lead to uncontrolled cell proliferation. In neurodegenerative disorders such as Alzheimer's disease altered neddylation can disrupt normal protein homeostasis contributing to neuron damage. UBA3's interaction with protein partners like cullins connects it to various pathological states highlighting its importance in disease development and progression.