Abcam, ab140509, Anti-RNA polymerase II RPB1 antibody

Size: 100µg
Goat Polyclonal RNA polymerase II RPB1 antibody. Suitable for WB, IP and reacts with Human, Mouse samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human POLR2A.
Key facts
Host species:Goat,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human POLR2A. The exact immunogen used to generate this antibody is proprietary information.P24928

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab140509 was affinity purified using an epitope specific to RNA polymerase II RPB1 immobilized on a solid support., Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: 99% Tris citrate/phosphate, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RNA polymerase II RPB1 also known as POLR2A or polymerase II is a protein that functions as a catalytic core in the transcription machinery. It plays an important role in transcribing DNA into mRNA essential for initiating protein production. RPB1 displays a molecular mass of approximately 220 kDa. It is ubiquitously expressed in eukaryotic cells signifying its extensive involvement across the cellular milieu.
Biological function summary
RNA polymerase II RPB1 orchestrates the assembly of the preinitiation complex collaborating with various transcription factors to enable accurate transcription initiation. It forms an important part of the RNA polymerase II complex which consists of twelve subunits in eukaryotes. This assembly facilitates the synthesis of pre-mRNA and snRNA which are paramount for proper gene expression and RNA processing.
Pathways
RNA polymerase II RPB1 integrates predominantly into the gene expression and regulation pathways including the transcription initiation and elongation processes. It operates alongside transcription factors like TFIIB and TFIIF which assist in positioning RPB1 correctly on the DNA template. It is also associated with signaling pathways such as MAPK which modulates cellular responses including growth and stress.
RNA polymerase II RPB1 has been implicated in conditions like Cockayne syndrome and cancer. Disruptions in its transcriptional functions can lead to defects in DNA repair and cell cycle control contributing to cancer progression. Links with proteins such as p53 a tumor suppressor involved in cell cycle regulation highlight its role in cancer-related pathways. Cockayne syndrome on the other hand arises due to impaired DNA damage response with defects in RNA polymerase II-linked transcription coupling repair.