Abcam, ab142773, Fluo-8 AM, green fluorescent calcium binding dye

Size: 1mg
Fluo-8 AM, a green fluorescent medium affinity calcium-binding dye binds to intracellular calcium (Kd = 390 nM). Fluorescence intensity increases upon Ca2+ binding.
Key facts
CAS number:1345980-40-6,
Form:SolidSee storage information,
Molecular weight:1046.94 g/mol,
Molecular formula:C50H50N2O23,
Nature:Synthetic,
Solubility:Soluble in DMSO,
Biological description:Medium affinity green fluorescent calcium binding dye. Binds to intracellular calcium (Kd = 390 nM). Fluorescence intensity increases upon Ca2+ binding. Cell-permeable.Fluo-8 (or Fluo-2 Medium Affinity) has been found to be brighter (1.5x) than Fluo-4 in cellular experiments. It offers improved cell loading and Ca2+ response while maintaining the convenient Fluo-3 and Fluo-4 spectral wavelength of maximum excitation at 490 nm and maximum emission at 520 nm. Fluo-8 loading can be performed at room temperature.,
Canonical smiles:CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)C5CCCCC5OCCOC1CC(CCC1N(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)C=2C4CCC(CC4OC3=CC(=O)C=CC=23)OCOC(C)=O,
InChi:InChI=1S/C50H50N2O23/c1-30(53)65-25-70-37-12-14-39-44(20-37)75-43-19-36(58)11-13-38(43)50(39)35-10-15-41(52(23-48(61)73-28-68-33(4)56)24-49(62)74-29-69-34(5)57)45(18-35)64-17-16-63-42-9-7-6-8-40(42)51(21-46(59)71-26-66-31(2)54)22-47(60)72-27-67-32(3)55/h6-15,18-20H,16-17,21-29H2,1-5H3,
InChiKey:BKYHTWFRZPRHNM-UHFFFAOYSA-N,
IUPAC Name:Bis(acetoxymethyl) 2,2'-((4-(6-(acetoxymethoxy)-3-oxo-3H-xanthen-9-yl)-2-(2-(bis(2-acetoxymethoxy)-2-oxoethyl)amino)phenoxy)ethoxy)phenyl)azanediyl)diacetate,
Excitation/Emission:Ex: 490nm, Em: 520nm

Product details:
Fluo-8® AM Working Solution: On the day of the experiment, either dissolve Fluo-8® AM in DMSO or thaw an aliquot of the indicator stock solution to room temperature. Prepare a dye working solution of 2 to 20 µM in a buffer of your choice (e.g., Hanks and Hepes buffer) with 0.04% Pluronic® F-127. For most cell lines, Fluo-8® AM at a final concentration of 4-5 μM is recommended. The exact concentration of indicators required for cell loading must be determined empirically.

Properties and Storage Information:
Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information-Store under desiccating conditions|The product can be stored for up to 12 months